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PMID: 1312463 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nuclear matrix attachment region organizes the Epstein-Barr viral plasmid in Raji cells into a single DNA domain.

The EMBO journal ·Vol. 11 ·No. 3 ·1992-03-00 ·Pages 1165-76

Jankelevich S, Kolman JL, Bodnar JW, Miller G

Abstract

The extrachromosomal Epstein-Barr virus (EBV) plasmid in the Burkitt lymphoma cell line, Raji, is stably associated with the nuclear matrix. This association is effected by a nuclear matrix attachment region (MAR) located in the BamHI C fragment of the viral genome; no other region of EBV DNA was found to be attached to the nuclear matrix with high affinity. The MAR was mapped to 5.2 kbp of DNA, greater than 80% of which is found on the nuclear matrix in unsynchronized cells expressing only viral latent cycle products. Thus the majority of viral plasmids in Raji cells use the same MAR. The MAR of EBV DNA contains the origin of latent viral DNA replication (oriP), the genes for the small viral RNAs (EBERs) and a 500 bp region immediately upstream of the EBER-1 gene. The clustering of the latent viral replication origin and the nearby enhancer and promoters for latent viral transcription on the nuclear matrix is likely to be crucial for regulation of the latent viral genome.

MeSH Terms
Burkitt Lymphoma/genetics DNA, Viral/genetics Electrophoresis, Agar Gel Genome, Viral Globins/genetics Herpesvirus 4, Human/genetics Humans Nuclear Matrix/metabolism Plasmids Restriction Mapping Tumor Cells, Cultured
Chemicals
DNA, Viral Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jankelevich S
Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510.
Kolman J L
Bodnar J W
Miller G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-03-00
Pages
1165-76
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556559
Subset
IM
Grants
NIAID NIH HHS · AI-00983-01 · United States
NIAID NIH HHS · AI07210 · United States
NCI NIH HHS · CA-16038 · United States
External Links
PubMed source PMC Full Text View journal details
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