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PMID: 1323713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multimerization of ICP0, a herpes simplex virus immediate-early protein.

Journal of virology ·Vol. 66 ·No. 9 ·1992-09-00 ·Pages 5598-602

Chen J, Panagiotidis C, Silverstein S

Abstract

ICP0, a herpes simplex virus immediate-early gene product, is a highly phosphorylated nuclear protein that is a potent activator of virus and host genes. Using biochemical and genetic assays employing plasmids encoding mutant forms of ICP0 and a recombinant adenovirus that expresses ICP0, we mutant forms of ICP0 and a recombinant adenovirus that expresses ICP0, we provide evidence that the protein multimerizes. Some mutant forms of ICP0 were transdominant and interfered with activation of a target reporter gene or with complementation of an ICP0-minus virus.

MeSH Terms
Adenoviridae/genetics Animals DNA Mutational Analysis Genetic Complementation Test Immediate-Early Proteins Protein Conformation Simplexvirus/genetics,metabolism Transcription, Genetic Ubiquitin-Protein Ligases Vero Cells Viral Interference Viral Proteins/genetics,isolation & purification,metabolism
Chemicals
Immediate-Early Proteins Viral Proteins Ubiquitin-Protein Ligases Vmw110 protein, Human herpesvirus 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen J
Department of Microbiology, Columbia University, New York, New York 10032.
Panagiotidis C
Silverstein S
References (41)
41 references, click to expand
  1. Herpes simplex virus type 1 ICP0 regulates expression of immediate-early, early, and late genes in productively infected cells.
    J Virol. 1992 May;66(5):2904-15 PMID: 1313909
  2. Evidence for a direct role for both the 175,000- and 110,000-molecular-weight immediate-early proteins of herpes simplex virus in the transactivation of delayed-early promoters.
    J Virol. 1985 Mar;53(3):751-60 PMID: 2983086
  3. Mutations in the activation region of herpes simplex virus regulatory protein ICP27 can be trans dominant.
    J Virol. 1991 Jul;65(7):3656-66 PMID: 1645788
  4. High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110.
    Nucleic Acids Res. 1991 Nov 25;19(22):6155-61 PMID: 1659686
  5. Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression.
    J Virol. 1991 May;65(5):2666-75 PMID: 1850040
  6. Characterization of DNA-protein complex formation in nuclear extracts with a sequence from the herpes simplex virus thymidine kinase gene.
    J Biol Chem. 1990 Jan 25;265(3):1648-57 PMID: 2153138
  7. Reactivation of latent herpes simplex virus by adenovirus recombinants encoding mutant IE-0 gene products.
    J Virol. 1990 Sep;64(9):4489-98 PMID: 2166826
  8. Construction and characterization of herpes simplex virus type 1 mutants with defined lesions in immediate early gene 1.
    J Gen Virol. 1989 May;70 ( Pt 5):1185-202 PMID: 2543774
  9. The herpes simplex virus virion host shutoff function.
    J Virol. 1989 Nov;63(11):4834-9 PMID: 2552156
  10. A detailed mutational analysis of Vmw110, a trans-acting transcriptional activator encoded by herpes simplex virus type 1.
    EMBO J. 1987 Jul;6(7):2069-76 PMID: 2820720
  11. Evidence of DNA: protein interactions that mediate HSV-1 immediate early gene activation by VP16.
    Genes Dev. 1988 Jun;2(6):730-42 PMID: 2843426
  12. Analysis of the functional domains of herpes simplex virus type 1 immediate-early polypeptide Vmw110.
    J Mol Biol. 1988 Jul 5;202(1):87-96 PMID: 2845096
  13. Adenovirus vector expressing functional herpes simplex virus ICP0.
    J Virol. 1988 Dec;62(12):4544-53 PMID: 2846870
  14. Isolation of herpes simplex virus regulatory protein ICP4 as a homodimeric complex.
    J Virol. 1985 Aug;55(2):329-37 PMID: 2991559
  15. Three trans-acting regulatory proteins of herpes simplex virus modulate immediate-early gene expression in a pathway involving positive and negative feedback regulation.
    J Virol. 1985 Dec;56(3):723-33 PMID: 2999428
  16. Characterization of the IE110 gene of herpes simplex virus type 1.
    J Gen Virol. 1986 Nov;67 ( Pt 11):2365-80 PMID: 3023529
  17. Host cell proteins bind to the cis-acting site required for virion-mediated induction of herpes simplex virus 1 alpha genes.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):71-5 PMID: 3025864
  18. Herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors.
    J Virol. 1987 Jul;61(7):2286-96 PMID: 3035226
  19. A transcriptional repressor encoded by BPV-1 shares a common carboxy-terminal domain with the E2 transactivator.
    Cell. 1987 Jul 3;50(1):69-78 PMID: 3036366
  20. How eukaryotic transcriptional activators work.
    Nature. 1988 Oct 20;335(6192):683-9 PMID: 3050531
  21. Negative effect of the transcriptional activator GAL4.
    Nature. 1988 Aug 25;334(6184):721-4 PMID: 3412449
  22. Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.
    Cell. 1981 May;24(2):555-65 PMID: 6263501
  23. Characterization of the herpes simplex virion-associated factor responsible for the induction of alpha genes.
    J Virol. 1983 May;46(2):371-7 PMID: 6302308
  24. Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0.
    J Virol. 1992 Apr;66(4):2261-7 PMID: 1312631
  25. Activation of the cellular transcription factor AP-1 in herpes simplex virus infected cells is dependent on the viral immediate-early protein ICPO.
    Nucleic Acids Res. 1991 Sep 25;19(18):4879-83 PMID: 1656381
  26. Mutational analysis of the sequence encoding ICP0 from herpes simplex virus type 1.
    Virology. 1991 Jan;180(1):207-20 PMID: 1845823
  27. trans-dominant inhibition of herpes simplex virus transcriptional regulatory protein ICP4 by heterodimer formation.
    J Virol. 1990 Aug;64(8):3916-26 PMID: 2164603
  28. E2 polypeptides encoded by bovine papillomavirus type 1 form dimers through the common carboxyl-terminal domain: transactivation is mediated by the conserved amino-terminal domain.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):510-4 PMID: 2536165
  29. Herpes simplex virus type 1 ICP0 plays a critical role in the de novo synthesis of infectious virus following transfection of viral DNA.
    J Virol. 1989 Nov;63(11):4579-89 PMID: 2552142
  30. A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence.
    Cell. 1988 Feb 12;52(3):425-34 PMID: 2830986
  31. Expression of a truncated viral trans-activator selectively impedes lytic infection by its cognate virus.
    Nature. 1988 Sep 29;335(6189):452-4 PMID: 2843776
  32. Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression.
    EMBO J. 1988 Dec 20;7(13):4231-8 PMID: 2854058
  33. Stimulation of expression of a herpes simplex virus DNA-binding protein by two viral functions.
    Mol Cell Biol. 1985 May;5(5):957-63 PMID: 2987684
  34. Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5265-9 PMID: 2991915
  35. Isolation and characterization of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate early polypeptide Vmw110.
    J Gen Virol. 1986 Dec;67 ( Pt 12):2571-85 PMID: 3025339
  36. Co-ordinate regulation of herpes simplex virus gene expression is mediated by the functional interaction of two immediate early gene products.
    J Mol Biol. 1986 Oct 5;191(3):395-409 PMID: 3029383
  37. Induction of a factor that binds to the polyoma virus A enhancer on differentiation of embryonal carcinoma cells.
    Nature. 1987 Jul 16-22;328(6127):254-6 PMID: 3037385
  38. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  39. Trans activation of transcription by herpes virus products: requirement for two HSV-1 immediate-early polypeptides for maximum activity.
    EMBO J. 1984 Dec 20;3(13):3135-41 PMID: 6098466
  40. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  41. Herpes simplex viruses with mutations in the gene encoding ICP0 are defective in gene expression.
    J Virol. 1992 May;66(5):2916-27 PMID: 1313910
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.9.5598-5602.1992
Published
1992-09-00
Pages
5598-602
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC289122
Subset
IM
Grants
NIGMS NIH HHS · GM38125 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.9.5598-5602.1992 PMC Full Text View journal details
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