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PMID: 1659686 Published · ppublish English Journal Article

High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110.

Nucleic acids research ·Vol. 19 ·No. 22 ·1991-11-25 ·Pages 6155-61

Everett RD, Orr A, Elliott M

Abstract

Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.

MeSH Terms
Animals Baculoviridae/genetics Blotting, Western Cattle Chromatography, Liquid DNA/metabolism DNA-Binding Proteins/metabolism Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Gene Expression Genes, Viral Genetic Vectors Immediate-Early Proteins Simplexvirus/metabolism Ubiquitin-Protein Ligases Viral Proteins/genetics,isolation & purification
Chemicals
DNA-Binding Proteins IE1 protein, Human herpesvirus 1 Immediate-Early Proteins Viral Proteins DNA Ubiquitin-Protein Ligases Vmw110 protein, Human herpesvirus 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Everett R D
MRC Virology Unit, Glasgow, UK.
Orr A
Elliott M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.22.6155
Published
1991-11-25
Pages
6155-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329111
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.22.6155 PMC Full Text View journal details
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