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PMID: 1329721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of lipid peroxidation and hydroxyl-radical generation by the contents of human atherosclerotic lesions.

The Biochemical journal ·Vol. 286 ( Pt 3) ·1992-09-15 ·Pages 901-5

Smith C, Mitchinson MJ, Aruoma OI, Halliwell B

Abstract

Lipid peroxidation within human arterial lesions is thought to play an important role in the development of atherosclerosis. Peroxidation can be accelerated by the presence of 'catalytic' iron or copper ions. Gruel samples from advanced atherosclerotic lesions in the abdominal aortae of human cadavers were tested for pro-oxidant properties. All samples contained bleomycin-detectable iron and phenanthroline-detectable copper. Almost all gruel samples stimulated peroxidation of rat liver microsomes, and this was usually inhibited by the iron-ion chelator desferrioxamine. Some samples stimulated formation of hydroxyl radicals from H2O2 in the presence of ascorbate, a reaction again inhibited by desferrioxamine. We conclude that the interior of human advanced atherosclerotic lesions is a highly pro-oxidant environment, and that the use of copper or iron ions to promote peroxidation of low-density lipoproteins in vitro may be a valid model for events in the arterial wall.

MeSH Terms
Animals Arteriosclerosis/metabolism Cations Copper/analysis Deferoxamine/pharmacology Humans Hydrogen Peroxide/metabolism Hydroxides/metabolism Hydroxyl Radical Iron/analysis Lipid Peroxidation/drug effects Microsomes, Liver/metabolism Rats Spectrophotometry, Atomic
Chemicals
Cations Hydroxides Hydroxyl Radical Copper Hydrogen Peroxide Iron Deferoxamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith C
Department of Biochemistry, Kings College, London, U.K.
Mitchinson M J
Aruoma O I
Halliwell B
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2860901
Published
1992-09-15
Pages
901-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132988
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2860901 PMC Full Text View journal details
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