Copper-phenanthroline-induced site-specific oxygen-radical damage to DNA. Detection of loosely bound trace copper in biological fluids.
Copper(II) ions, in the presence of 1,10-phenanthroline, O2 and a reducing agent, degrade DNA with the release of thiobarbituric-acid-reactive material. This reaction, dependent on the formation of oxygen radicals, was made the basis of a sensitive and specific assay for loosely bound copper in body fluids. When applied to certain extracellular fluids, trace amounts of copper could be detected in the lower micromolar range.
10 references, click to expand
-
The thiobarbituric acid-reactivity of bile pigments.Biochem Med. 1978 Feb;19(1):127-32 PMID: 623641
-
Serum copper and related variables in rheumatoid arthritis.Ann Rheum Dis. 1978 Feb;37(1):67-70 PMID: 629607
-
Oxygen-dependent cleavage of DNA by the 1,10-phenanthroline . cuprous complex. Inhibition of Escherichia coli DNA polymerase I.J Biol Chem. 1979 Dec 25;254(24):12269-72 PMID: 387784
-
Degradation of DNA by 1,-10-phenanthroline.Biochem Biophys Res Commun. 1980 Mar 13;93(1):264-70 PMID: 6445734
-
Copper salt-dependent hydroxyl radical formation. Damage to proteins acting as antioxidants.Biochim Biophys Acta. 1983 Aug 23;759(1-2):38-41 PMID: 6192847
-
Unusual copper-induced sensitization of the biological damage due to superoxide radicals.J Biol Chem. 1981 Dec 25;256(24):12632-5 PMID: 6273398
-
Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of 'free' iron in biological systems by using bleomycin-dependent degradation of DNA.Biochem J. 1981 Oct 1;199(1):263-5 PMID: 6175315
-
The role of the superoxide and hydroxyl radicals in the degradation of DNA and deoxyribose induced by a copper-phenanthroline complex.Biochem Pharmacol. 1982 Sep 1;31(17):2801-5 PMID: 6291545
-
Superoxide-dependent formation of hydroxyl radicals and lipid peroxidation in the presence of iron salts. Detection of 'catalytic' iron and anti-oxidant activity in extracellular fluids.Biochem J. 1982 Sep 15;206(3):605-9 PMID: 6293469
-
Degradation of deoxyribonucleic acid by a 1,10-phenanthroline-copper complex: the role of hydroxyl radicals.Biochemistry. 1980 Dec 23;19(26):5987-91 PMID: 7470443
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
