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PMID: 1337001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequential assignment of the backbone nuclei (1H, 15N and 13C) of c-H-ras p21 (1-166).GDP using a novel 4D NMR strategy.

Journal of biomolecular NMR ·Vol. 2 ·No. 6 ·1992-11-00 ·Pages 639-46

Campbell-Burk SL, Domaille PJ, Starovasnik MA, Boucher W, Laue ED

Abstract

The c-H-ras p21 protein is the product of the human ras proto-oncogene, a member of a ubiquitous eukaryotic gene family which is highly conserved in evolution. These proteins play an important role in the control of cellular growth. We report here the sequential assignment of the backbone nuclei in a truncated form of the 21-kD gene product, using our recently proposed 4D NMR strategy (Boucher et al., 1992). These assignments are the first step towards a full investigation of the structure, dynamics and interactions of wild-type and oncogenic ras p21 using NMR spectroscopy. Some of the data were presented at the 33rd ENC held at Asilomar, California, U.S.A., in April 1992.

MeSH Terms
Amino Acid Sequence Carbon Isotopes Guanosine Diphosphate/chemistry Humans Magnetic Resonance Spectroscopy/methods Molecular Sequence Data Nitrogen Isotopes Proto-Oncogene Mas Proto-Oncogene Proteins p21(ras)/chemistry Protons
Chemicals
Carbon Isotopes MAS1 protein, human Nitrogen Isotopes Proto-Oncogene Mas Protons Guanosine Diphosphate HRAS protein, human Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Campbell-Burk S L
DuPont Merck Pharmaceutical Co., Wilmington, DE 19880-0328.
Domaille P J
Starovasnik M A
Boucher W
Laue E D
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26 references, click to expand
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Article Info
Journal
Journal of biomolecular NMR
Abbr.
J Biomol NMR
ISSN
0925-2738
DOI
10.1007/BF02192852
Published
1992-11-00
Pages
639-46
Language
English
Region
Netherlands
NLM ID
9110829
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02192852 View journal details
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