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PMID: 136644 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.

Nucleic acids research ·Vol. 3 ·No. 10 ·1976-10-00 ·Pages 2665-75

Friedman J, Razin A

Abstract

In vivo inhibition of bacteriophage phiX174 DNA methylation by nicotinamide resulted in the accumulation of replicative intermediates with multiple-genome length single-stranded "tails". These abnormal replicative intermediates could not be chased into viral single-stranded circular DNA. The effect of nicotinamide on phage maturation and accumulation of abnormal replicative intermediates could be reversed by washing out the inhibitor. The results suggest that the single methyl group present in the viral DNA serves as a recognition site for a specific endonuclease, probably the gene A protein product, that is responsible for the excision of the single-stranded one-genome long viral DNA, before final maturation of the virus occurs.

MeSH Terms

Coliphages/metabolism DNA (Cytosine-5-)-Methyltransferases/antagonists & inhibitors,metabolism DNA Replication/drug effects DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Methyltransferases/metabolism Models, Biological Niacinamide/pharmacology Virus Replication/drug effects

Chemicals

DNA, Single-Stranded DNA, Viral Niacinamide Methyltransferases DNA (Cytosine-5-)-Methyltransferases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Friedman J

Razin A

References (11)

11 references, click to expand

Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide.
Nucleic Acids Res. 1975 Oct;2(10):1967-74 PMID: 127165
Cis-limited action of the gene-A product of bacteriophage phiX174 and the essential bacterial site (E. coli-electron microscopy-cis-acting protein-specifically-nicked RF).
Proc Natl Acad Sci U S A. 1972 Feb;69(2):475-9 PMID: 4551145
Filamentous bacterial viruses. VI. Role of fd gene 2 in deoxyribonucleic acid replication.
J Virol. 1972 Sep;10(3):384-91 PMID: 4561205
In vivo methylation of replicating bacteriophage phi chi174 DNA.
J Mol Biol. 1973 Aug 15;78(3):417-25 PMID: 4585324
DNA methylase induced by bacteriophage phiX174.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3773-5 PMID: 4590171
Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53 PMID: 4598307
Location of the 5-methylcytosine group on the bacteriophage phi X174 genome.
J Virol. 1974 Oct;14(4):872-7 PMID: 4609216
Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.
J Mol Biol. 1971 Nov 14;61(3):565-86 PMID: 5133115
The process of infection with bacteriophage phiX174. XXVI. Transfer of the parental DNA of bacteriophage phiX174 into progeny bacteriophage particles.
J Mol Biol. 1969 Feb 14;39(3):641-54 PMID: 5357215
The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.
J Mol Biol. 1969 Oct 28;45(2):237-63 PMID: 5367027
Structure of the DNA of bacteriophage phiX174. VII. Methylation.
J Mol Biol. 1970 Oct 28;53(2):251-9 PMID: 5493279

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.10.2665

Published

1976-10-00

Pages

2665-75

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343120

Subset

External Links

PubMed source DOI: 10.1093/nar/3.10.2665 PMC Full Text View journal details

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Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.

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