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PMID: 1379730 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 6988-92

Tuerk C, MacDougal S, Gold L

Abstract

High-affinity ligands of the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1) were isolated by the SELEX procedure (systematic evolution of ligands by exponential enrichment) from RNA populations randomized at 32 positions. Analysis of these ligands revealed a pseudoknot consensus with primary sequence bias at some positions. We demonstrated that at least one of the ligands inhibits cDNA synthesis by HIV reverse transcriptase but fails to inhibit other reverse transcriptases. These experiments highlight the power of SELEX to yield highly specific ligands that reduce the activity of target proteins. Such ligands may provide therapeutic reagents for viral and other diseases.

MeSH Terms
Antiviral Agents/pharmacology Base Sequence Drug Design HIV Reverse Transcriptase HIV-1/enzymology Ligands Molecular Sequence Data Nucleic Acid Conformation Protein Binding RNA, Antisense/metabolism,pharmacology Reverse Transcriptase Inhibitors
Chemicals
Antiviral Agents Ligands RNA, Antisense Reverse Transcriptase Inhibitors HIV Reverse Transcriptase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tuerk C
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
MacDougal S
Gold L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.6988
Published
1992-08-01
Pages
6988-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49630
Subset
IM
Grants
NIGMS NIH HHS · GM19963 · United States
NIGMS NIH HHS · GM28685 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.15.6988 PMC Full Text View journal details
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