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PMID: 2479543 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA.

The EMBO journal ·Vol. 8 ·No. 11 ·1989-11-00 ·Pages 3279-85

Barat C, Lullien V, Schatz O, Keith G, Nugeyre MT, Grüninger-Leitch F, Barré-Sinoussi F, LeGrice SF, Darlix JL

Abstract

The virion cores of the replication competent type 1 human immunodeficiency virus (HIV-1), a retrovirus, contain and RNA genome associated with nucleocapsid (NC) and reverse transcriptase (RT p66/p51) molecules. In vitro reconstructions of these complexes with purified components show that NC is required for efficient annealing of the primer tRNALys,3. In the absence of NC, HIV-1 RT is unable to retrotranscribe the viral RNA template from the tRNA primer. We demonstrate that the HIV-1 RT p66/p51 specifically binds to its cognate primer tRNALys,3 even in the presence of a 100-fold molar excess of other tRNAs. Cross-linking analysis of this interaction locates the contact site to a region within the heavily modified anti-codon domain of tRNALys,3.

MeSH Terms
Anticodon/metabolism Base Sequence Binding Sites Capsid/metabolism Cell Line Electrophoresis HIV-1/enzymology,genetics Humans Immunoblotting Molecular Sequence Data Nucleic Acid Conformation RNA, Transfer/metabolism RNA, Transfer, Amino Acid-Specific/metabolism RNA, Transfer, Lys/genetics,metabolism RNA-Directed DNA Polymerase/metabolism Viral Core Proteins/metabolism
Chemicals
Anticodon RNA, Transfer, Amino Acid-Specific RNA, Transfer, Lys Viral Core Proteins RNA, Transfer RNA-Directed DNA Polymerase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Barat C
Labo-Retro Centre de Recherche de Biochimie et Génétique Cellulaires du CNRS, Toulouse, France.
Lullien V
Schatz O
Keith G
Nugeyre M T
Grüninger-Leitch F
Barré-Sinoussi F
LeGrice S F
Darlix J L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-11-00
Pages
3279-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401457
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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