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PMID: 3553862 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The bacteriophage T4 dexA gene: sequence and analysis of a gene conditionally required for DNA replication.

Molecular & general genetics : MGG ·Vol. 206 ·No. 1 ·1987-01-00 ·Pages 24-34

Gauss P, Gayle M, Winter RB, Gold L

Abstract

We have cloned and sequenced a bacteriophage T4 EcoRI fragment that complements T4 del (39-56) infections of an optA defective Escherichia coli strain. Bacteria containing this recombinant plasmid synthesize two new proteins with molecular weights of 9 and 26 kilodaltons. We have identified the gene encoding the 26 kilodalton protein as essential for T4 infections of optA defective E. coli. Genetic and biochemical results are consistent with the identification of this protein as the product of the dexA gene, which encodes a 3' to 5' exonuclease.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Coliphages/genetics DNA Replication Escherichia coli/genetics Genes Genes, Viral Genetic Complementation Test Genotype Mutation Plasmids Transcription, Genetic Viral Proteins/genetics
Chemicals
Viral Proteins dexA protein, T4 phage
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gauss P
Gayle M
Winter R B
Gold L
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38 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00326532
Published
1987-01-00
Pages
24-34
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM19963 · United States
NIGMS NIH HHS · GM28685 · United States
Databases
GENBANK
K03112, X04834
External Links
PubMed source DOI: 10.1007/BF00326532
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