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PMID: 6300866 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Bacterial and phage mutations that reveal helix-unwinding activities required for bacteriophage T4 DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 6 ·1983-03-00 ·Pages 1669-73

Gauss P, Doherty DH, Gold L

Abstract

An Escherichia coli strain with a mutation in the optA gene restricts the growth of bacteriophage T4 strains partially defective in gene 43 (DNA polymerase) or missing gene dda (DNA-dependent ATPase). The mutations in the dda gene inactivate a DNA-dependent ATPase that has been shown to have DNA helicase activity in vitro. We show that the restriction of phage growth after infection of the optA bacterium is the result of a block in DNA replication. We infer that the block arises from a defect in DNA unwinding.

MeSH Terms
DNA Helicases/physiology DNA Replication DNA, Viral/biosynthesis DNA-Directed DNA Polymerase/genetics Escherichia coli/genetics Genes, Viral Mutation T-Phages/genetics Virus Replication
Chemicals
DNA, Viral DNA-Directed DNA Polymerase DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gauss P
Doherty D H
Gold L
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.6.1669
Published
1983-03-00
Pages
1669-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393664
Subset
IM
Grants
NIGMS NIH HHS · GM06459 · United States
NIGMS NIH HHS · GM19963 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.6.1669 PMC Full Text View journal details
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