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PMID: 1383233 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Introns excised from the Delta primary transcript are localized near sites of Delta transcription.

The Journal of cell biology ·Vol. 119 ·No. 3 ·1992-11-00 ·Pages 503-12

Kopczynski CC, Muskavitch MA

Abstract

Introns excised from the primary transcript of Delta (Dl), a Drosophila neurogenic gene, accumulate to unusually high levels in embryos. High resolution in situ hybridization reveals a striking localization of the excised introns to two foci per embryonic nucleus. The number of foci can be altered by varying the number of Dl genes present in the embryonic nucleus, suggesting that the excised introns are localized near sites of Dl transcription. This conclusion is supported by the observation that larval and imaginal disc nuclei containing two copies of Dl exhibit only one focus of intron accumulation, as expected for nuclei in which homologous chromosomes are paired. Interestingly, the excised introns do not appear to diffuse away from the foci until late prophase, at which time the foci disperse into numerous small dots of hybridization. These results suggest that the excised Dl introns may be associated with a structural element within the nucleus that is dissociated during cell division.

Related Genes
DI
MeSH Terms
Animals Base Sequence Cell Nucleus/physiology Chromosomes/physiology Drosophila melanogaster/embryology,genetics Embryo, Nonmammalian/physiology Exons In Situ Hybridization Interphase Introns Mitosis Molecular Sequence Data Nervous System/embryology Nervous System Physiological Phenomena Oligodeoxyribonucleotides Polymerase Chain Reaction RNA/genetics,isolation & purification Transcription, Genetic
Chemicals
Oligodeoxyribonucleotides RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kopczynski C C
Department of Biology, Indiana University, Bloomington 47405.
Muskavitch M A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.3.503
Published
1992-11-00
Pages
503-12
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289664
Subset
IM
Grants
NIGMS NIH HHS · GM33291 · United States
External Links
PubMed source DOI: 10.1083/jcb.119.3.503 PMC Full Text View journal details
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