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PMID: 1400584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA.

The Journal of cell biology ·Vol. 119 ·No. 2 ·1992-10-00 ·Pages 427-38

Rodríguez Fernández JL, Geiger B, Salomon D, Sabanay I, Zöller M, Ben-Ze'ev A

Abstract

Transfection of chicken vinculin cDNA into two tumor cell lines expressing diminished levels of the endogenous protein, brought about a drastic suppression of their tumorigenic ability. The SV-40-transformed Balb/c 3T3 line (SVT2) contains four times less vinculin than the parental 3T3 cells, and the rat adenocarcinoma BSp73ASML has no detectable vinculin. Restoration of vinculin in these cells, up to the levels found in 3T3 cells, resulted in an apparent increase in substrate adhesiveness, a decrease in the ability to grow in soft agar, and suppression of their capacity to develop tumors after injection into syngeneic hosts or nude mice. These results suggest that vinculin, a cytoplasmic component of cell-matrix and cell-cell adhesions, may have a major suppressive effect on the transformed phenotype.

MeSH Terms
3T3 Cells Adenocarcinoma Animals Cell Adhesion/drug effects Cell Division/drug effects Cell Line, Transformed Cell Transformation, Neoplastic/drug effects,genetics Cell Transformation, Viral/genetics Chickens DNA, Single-Stranded/genetics,pharmacology Mice Mice, Nude Neoplasms, Experimental Transfection Vinculin/genetics,pharmacology
Chemicals
DNA, Single-Stranded Vinculin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rodríguez Fernández J L
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
Geiger B
Salomon D
Sabanay I
Zöller M
Ben-Ze'ev A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.2.427
Published
1992-10-00
Pages
427-38
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289642
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.119.2.427 PMC Full Text View journal details
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