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PMID: 1409621 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Characterization of Plasmodium falciparum sporozoite surface protein 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 19 ·1992-10-01 ·Pages 9176-80

Rogers WO, Malik A, Mellouk S, Nakamura K, Rogers MD, Szarfman A, Gordon DM, Nussler AK, Aikawa M, Hoffman SL

Abstract

Immunization of mice with Plasmodium yoelii sporozoite surface protein 2 (PySSP2) and circumsporozoite protein protects completely against P. yoelii. The amino acid sequence of PySSP2 suggested that the thrombospondin-related anonymous protein (TRAP) [Robson, K. J. H., Hall, J. R. S., Jennings, M. W., Harris, T. J. R., Marsh, K., Newbold, C. I., Tate, V. E. & Weatherall, D. J. (1988) Nature (London) 335, 79-82] is the Plasmodium falciparum homolog of PySSP2. We report data confirming that TRAP is P. falciparum SSP2 (PfSSP2). Murine antibodies against recombinant PfSSP2 identify a 90-kDa protein in extracts of P. falciparum sporozoites, recognize sporozoites and infected hepatocytes by immunofluorescence, localize PfSSP2 to the sporozoite micronemes by immunoelectron microscopy and to the surface membrane by live immunofluorescence, and inhibit sporozoite invasion and development in hepatocytes in vitro. Human volunteers immunized with irradiated sporozoites and protected against malaria develop antibody and proliferative T-cell responses to PfSSP2, suggesting that, like PySSP2, PfSSP2 is a target of protective immunity, and supporting inclusion of PfSSP2 in a multicomponent malaria vaccine.

MeSH Terms
Amino Acid Sequence Animals Antigens, Protozoan/genetics Base Sequence Blotting, Western Cloning, Molecular Lymphocyte Activation Microscopy, Immunoelectron Molecular Sequence Data Oligodeoxyribonucleotides Plasmodium falciparum/genetics,ultrastructure Polymerase Chain Reaction/methods Protozoan Proteins/genetics,ultrastructure Repetitive Sequences, Nucleic Acid T-Lymphocytes/immunology
Chemicals
Antigens, Protozoan Oligodeoxyribonucleotides Protozoan Proteins thrombospondin-related adhesive protein, protozoan sporozoite-surface protein 2, Plasmodium yoelli
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rogers W O
Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889.
Malik A
Mellouk S
Nakamura K
Rogers M D
Szarfman A
Gordon D M
Nussler A K
Aikawa M
Hoffman S L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.19.9176
Published
1992-10-01
Pages
9176-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50088
Subset
IM
Databases
GENBANK
M94013
External Links
PubMed source DOI: 10.1073/pnas.89.19.9176 PMC Full Text View journal details
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