DNA translocations contribute to chromosome length polymorphisms in Candida albicans.

Current genetics ·Vol. 22 ·No. 2 ·1992-08-00 ·Pages 93-100

Thrash-Bingham C, Gorman JA

Abstract

Rotating-gel electrophoresis and DNA hybridization were used to compare the electrophoretic karyotype of six Candida albicans isolates. The hybridization pattern for 22 cloned sequences, including eight previously unmapped genes, indicates that there are eight pair of homologous chromosomes in each strain. However, since homologous chromosomes can differ in length, it is possible to resolve more than eight bands in some strains. The mapping data demonstrate that linkage groups are generally conserved suggesting that, in spite of gross karyotype differences, there is an underlying similarity in the genome organization of different isolates. The hybridization data also provide direct evidence that DNA translocations and reciprocal translocations contribute to chromosome length polymorphisms in C. albicans.

Related Genes

ADE1 ADE2 BEN1 DFR1 ENO1 ERG1 ERG16 ERG7 GAL1 HEM3 HIS3 LEU2 LYS2 PMA1 TRP1 URA3

MeSH Terms

Candida albicans/genetics Chromosome Banding Chromosomes, Fungal/ultrastructure DNA, Fungal/genetics Electrophoresis, Gel, Pulsed-Field Genes, Fungal Genetic Markers Karyotyping/methods Nucleic Acid Hybridization Polymorphism, Genetic Translocation, Genetic

Chemicals

DNA, Fungal Genetic Markers

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thrash-Bingham C

Department of Microbial Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543-4000.

Gorman J A

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00351467

Published

1992-08-00

Pages

93-100

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00351467 View journal details

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DNA translocations contribute to chromosome length polymorphisms in Candida albicans.

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