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PMID: 1429432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutants carrying conditionally lethal mutations in outer membrane genes omsA and firA (ssc) are phenotypically similar, and omsA is allelic to firA.

Journal of bacteriology ·Vol. 174 ·No. 22 ·1992-11-00 ·Pages 7090-7

Vuorio R, Vaara M

Abstract

We have previously identified the gene (the ssc gene) defective in the thermosensitive and antibiotic-supersusceptible outer membrane permeability mutant SS-C of Salmonella typhimurium and shown that this gene is analogous to the Escherichia coli gene firA (L. Hirvas, P. Koski, and M. Vaara, EMBO J. 10:1017-1023, 1991). Others have tentatively implicated firA in a different function, mRNA synthesis. Here we report that the defect in the thermosensitive outer membrane omsA mutant of E. coli (T. Tsuruoka, M. Ito, S. Tomioka, A. Hirata, and M. Matsuhashi, J. Bacteriol. 170:5229-5235, 1988) is due to a mutation in firA; this mutation changed codon 271 from serine to asparagine. The omsA-induced phenotype was completely reverted by plasmids containing wild-type firA or ssc. Plasmids carrying the omsA allele, or an identical mutant allele prepared by localized mutagenesis, under the control of lac elicited partial complementation. Transcomplementation studies with plasmids carrying various mutant alleles of the S. typhimurium gene indicated that the ability of these plasmids to complement the omsA mutation was similar to their ability to complement the ssc mutation. The antibiotic-supersusceptible phenotype of the omsA mutant closely resembled that of the ssc mutant, i.e., the omsA mutant was supersusceptible to hydrophobic antibiotics and large-peptide antibiotics against which the intact outer membrane is an effective permeability barrier. As previously demonstrated with the omsA mutant, the outer membrane of the ssc mutant became selectively ruptured after incubation for 1 h at the growth-nonpermitting temperature; 82% of the periplasmic beta-lactamase and less than 3% of the cytoplasmic marker enzyme were released into the medium. All of these findings are consistent with our concept that firA is an essential gene involved in generation of the outer membrane.

Related Genes
MeSH Terms
Alleles Ampicillin Resistance/genetics Anti-Bacterial Agents/pharmacology Bacterial Outer Membrane Proteins/genetics Base Sequence Blotting, Southern DNA, Neoplasm/genetics,isolation & purification Escherichia coli/drug effects,genetics,growth & development Genes, Bacterial Genes, Lethal Hot Temperature Kinetics Microbial Sensitivity Tests Molecular Sequence Data Mutagenesis, Site-Directed Oligonucleotide Probes Phenotype Plasmids Salmonella typhimurium/drug effects,genetics,growth & development Species Specificity beta-Lactamases/genetics,metabolism
Chemicals
Anti-Bacterial Agents Bacterial Outer Membrane Proteins DNA, Neoplasm Oligonucleotide Probes beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vuorio R
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Vaara M
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1992-11-00
Pages
7090-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207397
Subset
IM
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