Home LiteratureArticle Details
PMID: 1435724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

FinOP repression of the F plasmid involves extension of the half-life of FinP antisense RNA by FinO.

Molecular & general genetics : MGG ·Vol. 235 ·No. 1 ·1992-10-00 ·Pages 131-9

Lee SH, Frost LS, Paranchych W

Abstract

The transfer operon of the F plasmid is positively regulated by the traJ gene product, expression of which, in turn, is regulated by both an antisense RNA, FinP, and the FinO protein (the FinOP system). A finP- F plasmid, pSFL20, was constructed by site-directed mutagenesis and was found to produce wild-type levels of pili encoded by the transfer operon. Transcription of the traJ gene was decreased by a factor of 3-5 fold in the presence of FinOP with no accumulation of a stable RNA duplex between the FinP RNA and the portion of the traJ mRNA which is complementary to finP. Stabilization of FinP RNA by FinO occurs in the absence of traJ transcripts, suggesting that FinO may interact directly with FinP to prevent its degradation.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,metabolism Bacterial Proteins/metabolism Base Sequence Cloning, Molecular DNA, Bacterial Endoribonucleases/metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins F Factor Gene Expression Regulation, Bacterial Half-Life Molecular Sequence Data Mutagenesis, Site-Directed Operon Promoter Regions, Genetic RNA, Antisense/metabolism RNA-Binding Proteins Repressor Proteins Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Bacterial Escherichia coli Proteins RNA, Antisense RNA-Binding Proteins Repressor Proteins TraJ protein, E coli TraJ protein, bacteria FinO protein, E coli Endoribonucleases ribonuclease E
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee S H
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Frost L S
Paranchych W
References (32)
32 references, click to expand
  1. Sense and antisense transcripts of traM, a conjugal transfer gene of the antibiotic resistance plasmid R100.
    Mol Microbiol. 1989 Apr;3(4):561-70 PMID: 2474740
  2. The site of action of the F transfer inhibitor.
    Mol Gen Genet. 1973 Dec 31;127(4):307-16 PMID: 4594003
  3. Repression and derepression of conjugation of plasmid R1 by wild-type and mutated finP antisense RNA.
    Mol Microbiol. 1991 Jan;5(1):77-87 PMID: 1707476
  4. Plasmid expression vector using the lambda late promoter.
    Plasmid. 1986 May;15(3):242-4 PMID: 2940611
  5. Regulation of the F plasmid traY promoter in Escherichia coli by host and plasmid factors.
    J Mol Biol. 1991 Mar 5;218(1):119-28 PMID: 2002497
  6. finP and fisO mutations in FinP anti-sense RNA suggest a model for FinOP action in the repression of bacterial conjugation by the Flac plasmid JCFL0.
    Mol Gen Genet. 1989 Jul;218(1):152-60 PMID: 2476653
  7. Nucleotide sequences of five IncF plasmid finP alleles.
    J Bacteriol. 1986 Aug;167(2):754-7 PMID: 2426248
  8. The F plasmid origin of transfer: DNA sequence of wild-type and mutant origins and location of origin-specific nicks.
    EMBO J. 1984 May;3(5):1175-80 PMID: 6329741
  9. Inactivation of the ribonucleic acid-processing enzyme ribonuclease E blocks cell division.
    J Bacteriol. 1981 Apr;146(1):128-32 PMID: 6163761
  10. Specificities of IncF plasmid conjugation genes.
    Genet Res. 1986 Feb;47(1):1-11 PMID: 3514378
  11. Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.
    EMBO J. 1988 Nov;7(11):3601-7 PMID: 3061803
  12. The conjugation system of F, the fertility factor of Escherichia coli.
    Annu Rev Genet. 1986;20:593-624 PMID: 2880557
  13. Regulation of the F plasmid traY promoter in Escherichia coli K12 as a function of sequence context.
    J Mol Biol. 1991 Jul 20;220(2):271-9 PMID: 1906941
  14. Structure and function of conjugative pili: inducible synthesis of functional F pili by Escherichia coli K-12 containing a lac-tra operon fusion.
    J Bacteriol. 1989 Feb;171(2):650-6 PMID: 2563366
  15. Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.
    J Bacteriol. 1987 Feb;169(2):619-23 PMID: 3027040
  16. Nucleotide sequence of the tra YALE region from IncFV plasmid pED208.
    J Bacteriol. 1986 Nov;168(2):990-8 PMID: 2877970
  17. Promoters in the transfer region of plasmid F.
    Basic Life Sci. 1985;30:605-14 PMID: 3893413
  18. Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor.
    Mol Gen Genet. 1971;111(3):256-64 PMID: 5563936
  19. A stable core region of the tra operon mRNA of plasmid R1-19.
    Nucleic Acids Res. 1989 Feb 25;17(4):1283-98 PMID: 2564189
  20. Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ.
    Mol Gen Genet. 1982;188(3):513-8 PMID: 6298579
  21. The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli.
    Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):1-5 PMID: 1846032
  22. A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.
    Gene. 1981 Dec;16(1-3):21-6 PMID: 6282692
  23. The F plasmid carries an IS3 insertion within finO.
    J Gen Microbiol. 1986 Dec;132(12):3269-75 PMID: 2821161
  24. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli.
    Gene. 1987;51(2-3):255-67 PMID: 3110013
  25. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  26. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.
    J Bacteriol. 1971 May;106(2):529-38 PMID: 4929865
  27. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  28. Cloning, mapping, and sequencing of plasmid R100 traM and finP genes.
    J Bacteriol. 1986 Jul;167(1):336-45 PMID: 3522549
  29. Removal of a terminator structure by RNA processing regulates int gene expression.
    J Mol Biol. 1984 Jun 15;176(1):39-53 PMID: 6234400
  30. Regulation of the F conjugation genes studied by hybridization and tra-lacZ fusion.
    J Mol Biol. 1983 Jul 25;168(1):103-22 PMID: 6192244
  31. Comparative biochemical studies on F and EDP208 conjugative pili.
    J Bacteriol. 1980 Jan;141(1):333-41 PMID: 6101592
  32. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00286190
Published
1992-10-00
Pages
131-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00286190
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2