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PMID: 14515327 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord.

Glia ·Vol. 44 ·No. 2 ·2003-11-00 ·Pages 111-8

Inoue M, Honmou O, Oka S, Houkin K, Hashi K, Kocsis JD

Abstract

The remyelinating potential of autologous bone marrow cells was studied after direct injection and following intravenous injection into rats with a demyelinated lesion in the spinal cord. Both focal and intravenous injections of acutely isolated mononuclear bone marrow cell fractions resulted in varying degrees of remyelination. Suspensions of bone marrow cells collected from the same rat were delivered at varied concentrations (10(2) to 10(5) for direct injection and 10(4) to 10(7) for i.v. injections). The lesions were examined histologically 3 weeks after transplantation. Light microscopic examination revealed remyelination in the dorsal funiculus with both injection protocols, but the extent of remyelination was proportional to the number of injected cells. To attain the same relative density of remyelination achieved by direct injection, intravenous administration of cells required delivery of substantially more cells (two orders of magnitude). However, the availability of autologous bone marrow cells in large number and the potential for systemically delivering cells to target lesion areas without neurosurgical intervention suggest the potential utility of intravenous cell delivery as a prospective therapeutic approach in demyelinating disease.

MeSH Terms
Animals Bone Marrow Transplantation Cell Transplantation/methods Demyelinating Diseases/pathology,therapy Female Injections, Intravenous Nerve Fibers, Myelinated/transplantation Rats Rats, Wistar Spinal Cord/pathology,transplantation Transplantation, Autologous
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Inoue Michio
Department of Neurosurgery, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan.
Honmou Osamu
Oka Shinichi
Houkin Kiyohiro
Hashi Kazuo
Kocsis Jeffery D
References (34)
34 references, click to expand
  1. Multipotent neural precursors can differentiate toward replacement of neurons undergoing targeted apoptotic degeneration in adult mouse neocortex.
    Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11663-8 PMID: 9326667
  2. Muscle regeneration by bone marrow-derived myogenic progenitors.
    Science. 1998 Mar 6;279(5356):1528-30 PMID: 9488650
  3. Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats--similarities to astrocyte grafts.
    Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3908-13 PMID: 9520466
  4. Bone marrow transplantation for autoimmune diseases.
    Acta Haematol. 1998;99(3):116-32 PMID: 9587393
  5. Role of adhesion molecules in the homing and mobilization of murine hematopoietic stem and progenitor cells.
    Blood. 1998 Aug 1;92(3):894-900 PMID: 9680357
  6. Stem cell homing: rolling, crawling, and nesting.
    Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15155-7 PMID: 9860935
  7. Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation.
    J Cell Biochem. 1999 Mar 15;72(4):570-85 PMID: 10022616
  8. Targeting of marrow-derived astrocytes to the ischemic brain.
    Neuroreport. 1999 Apr 26;10(6):1289-92 PMID: 10363941
  9. Embryonic stem cell-derived glial precursors: a source of myelinating transplants.
    Science. 1999 Jul 30;285(5428):754-6 PMID: 10427001
  10. Soluble intercelluar adhesion molecule-1, E-selectin, vascular cell adhesion molecule-1 and von Willebrand factor in stroke.
    Blood Coagul Fibrinolysis. 1999 Jul;10(5):277-84 PMID: 10456619
  11. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains.
    Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10711-6 PMID: 10485891
  12. Isolation, characterization, and use of stem cells from the CNS.
    Annu Rev Neurosci. 1995;18:159-92 PMID: 7605059
  13. The use of cultured autologous Schwann cells to remyelinate areas of persistent demyelination in the central nervous system.
    J Neurol Sci. 1985 Sep;70(2):207-23 PMID: 4056820
  14. Precursor cells of mechanocytes.
    Int Rev Cytol. 1976;47:327-59 PMID: 11195
  15. Remyelination of the spinal cord following intravenous delivery of bone marrow cells.
    Glia. 2002 Sep;39(3):229-36 PMID: 12203389
  16. Remyelination of the rat spinal cord by transplantation of identified bone marrow stromal cells.
    J Neurosci. 2002 Aug 1;22(15):6623-30 PMID: 12151541
  17. Efficient recolonisation of progenitor-depleted areas of the CNS by adult oligodendrocyte progenitor cells.
    Glia. 2002 Mar 15;37(4):307-13 PMID: 11870870
  18. Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery.
    Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2199-204 PMID: 11854516
  19. Intravenous administration of human umbilical cord blood reduces behavioral deficits after stroke in rats.
    Stroke. 2001 Nov;32(11):2682-8 PMID: 11692034
  20. Myelination following transplantation of EGF-responsive neural stem cells into a myelin-deficient environment.
    Exp Neurol. 1997 Sep;147(1):84-95 PMID: 9294405
  21. Marrow stromal cells as stem cells for nonhematopoietic tissues.
    Science. 1997 Apr 4;276(5309):71-4 PMID: 9082988
  22. Schwann cell-like myelination following transplantation of an olfactory bulb-ensheathing cell line into areas of demyelination in the adult CNS.
    Glia. 1996 Jul;17(3):217-24 PMID: 8840163
  23. Cytokines and adhesion molecules in stroke and related diseases.
    J Neurol Sci. 1996 May;137(2):69-78 PMID: 8782158
  24. Host regulation of glial markers in intrastriatal grafts of conditionally immortalized neural stem cell lines.
    Neuroreport. 1996 Mar 22;7(4):847-52 PMID: 8724658
  25. Restoration of normal conduction properties in demyelinated spinal cord axons in the adult rat by transplantation of exogenous Schwann cells.
    J Neurosci. 1996 May 15;16(10):3199-208 PMID: 8627358
  26. Cytokine-regulated expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in human microvascular endothelial cells.
    J Immunol. 1996 Apr 1;156(7):2558-65 PMID: 8786319
  27. The temporal profiles of ICAM-1 protein and mRNA expression after transient MCA occlusion in the rat.
    Brain Res. 1995 Jun 5;682(1-2):182-8 PMID: 7552309
  28. Adult rat and human bone marrow stromal cells differentiate into neurons.
    J Neurosci Res. 2000 Aug 15;61(4):364-70 PMID: 10931522
  29. Transplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord.
    Glia. 2000 May;30(3):209-18 PMID: 10756071
  30. From marrow to brain: expression of neuronal phenotypes in adult mice.
    Science. 2000 Dec 1;290(5497):1775-9 PMID: 11099418
  31. Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow.
    Science. 2000 Dec 1;290(5497):1779-82 PMID: 11099419
  32. Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons.
    Glia. 2001 Jul;35(1):26-34 PMID: 11424189
  33. Treatment of autoimmune diseases by hematopoietic stem cell transplantation.
    Exp Hematol. 2001 Jun;29(6):661-9 PMID: 11378260
  34. Transplantation of clonal neural precursor cells derived from adult human brain establishes functional peripheral myelin in the rat spinal cord.
    Exp Neurol. 2001 Jan;167(1):27-39 PMID: 11161590
Article Info
Journal
Glia
Abbr.
Glia
ISSN
0894-1491
DOI
10.1002/glia.10285
Published
2003-11-00
Pages
111-8
Language
English
Region
United States
NLM ID
8806785
PMCID
PMC2605389
Subset
IM
Grants
NINDS NIH HHS · R01 NS043432 · United States
NINDS NIH HHS · R01 NS043432-05 · United States
NINDS NIH HHS · NS043432 · United States
External Links
PubMed source DOI: 10.1002/glia.10285 PMC Full Text View journal details
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