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PMID: 14532108 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling.

The EMBO journal ·Vol. 22 ·No. 20 ·2003-10-15 ·Pages 5347-57

Virmani T, Han W, Liu X, Südhof TC, Kavalali ET

Abstract

The speed of synaptic vesicle recycling determines the efficacy of neurotransmission during repetitive stimulation. Synaptotagmins are synaptic C(2)-domain proteins that are involved in exocytosis, but have also been linked to endocytosis. We now demonstrate that upon expression in transfected neurons, a short splice variant of synaptotagmin 7 that lacks C(2)-domains accelerates endocytic recycling of synaptic vesicles, whereas a longer splice variant that contains C(2)-domains decelerates recycling. These results suggest that alternative splicing of synaptotagmin 7 acts as a molecular switch, which targets vesicles to fast and slow recycling pathways.

MeSH Terms
Alternative Splicing Animals Animals, Newborn Calcium-Binding Proteins Cells, Cultured Genetic Variation Hippocampus Membrane Glycoproteins/genetics,physiology Membrane Potentials/physiology Microscopy, Electron Nerve Tissue Proteins/genetics,physiology Neurons/cytology,physiology Rats Rats, Sprague-Dawley Receptors, Cell Surface/genetics Recombinant Proteins/metabolism Synapses/ultrastructure Synaptic Vesicles/physiology,ultrastructure Synaptotagmins
Chemicals
Calcium-Binding Proteins Membrane Glycoproteins Nerve Tissue Proteins Receptors, Cell Surface Recombinant Proteins Syt7 protein, rat Synaptotagmins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Virmani Tuhin
Center for Basic Neuroscience, Department of Molecular Genetics, The University of Texas Southwestern Medical Center, Dallas, TX 75390-9111, USA.
Han Weiping
Liu Xinran
Südhof Thomas C
Kavalali Ege T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg514
Published
2003-10-15
Pages
5347-57
Language
English
Region
England
NLM ID
8208664
PMCID
PMC213769
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg514 PMC Full Text View journal details
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