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PMID: 1454810 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 23 ·1992-12-01 ·Pages 11292-6

Tschochner H, Sayre MH, Flanagan PM, Feaver WJ, Kornberg RD

Abstract

Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.

Related Genes
ADC1 ADH1 GAL10 PYK1 SUA7
MeSH Terms
Amino Acid Sequence Fungal Proteins/isolation & purification,metabolism Gene Expression Regulation, Fungal Molecular Sequence Data Peptide Fragments/chemistry Promoter Regions, Genetic RNA Polymerase II/metabolism RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Schizosaccharomyces/genetics Transcription Factor TFIIA Transcription Factors/isolation & purification,metabolism Transcription, Genetic
Chemicals
Fungal Proteins Peptide Fragments RNA, Messenger Transcription Factor TFIIA Transcription Factors RNA Polymerase II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tschochner H
Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, CA 94305.
Sayre M H
Flanagan P M
Feaver W J
Kornberg R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.23.11292
Published
1992-12-01
Pages
11292-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50536
Subset
IM
Grants
NIGMS NIH HHS · GM36659 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.23.11292 PMC Full Text View journal details
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