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PMID: 14551791 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Collection of nasal secretions for immunological analysis.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery ·Vol. 261 ·No. 5 ·2004-05-00 ·Pages 242-6

Watelet JB, Gevaert P, Holtappels G, Van Cauwenberge P, Bachert C

Abstract

The biochemical analysis of nasal secretions has become essential in the study of nasal or sinus diseases and the monitoring of medical and surgical treatment. The nasal fluid greatly reflects the inflammatory activity within the nasal mucosa. This paper discusses techniques for nasal fluid collection described before and proposes a new approach for the collection and calculation of nasal secretions based on sinus packs. The method is non-invasive, well standardized and reproducible and therefore may serve as a valid tool for future investigations.

MeSH Terms
Adolescent Adult Aged Case-Control Studies Enzyme-Linked Immunosorbent Assay Female Humans Immunity, Mucosal Male Middle Aged Nasal Lavage Fluid/immunology Nasal Mucosa/immunology,metabolism Nasal Polyps/immunology,pathology Nasal Provocation Tests/methods Paranasal Sinuses/metabolism Reference Values Reproducibility of Results Sensitivity and Specificity Specimen Handling
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Watelet Jean-Baptiste
Department of Otorhinolaryngology, Ghent University Hospital, Ghent, Belgium. [email protected]
Gevaert Philippe
Holtappels Gabriele
Van Cauwenberge Paul
Bachert Claus
References (25)
25 references, click to expand
  1. Norm values for eosinophil cationic protein in nasal secretions: influence of specimen collection.
    Clin Exp Allergy. 1999 Mar;29(3):367-74 PMID: 10202345
  2. The significance and technical aspects of quantitative measurements of inflammatory mediators in allergic rhinitis.
    Asian Pac J Allergy Immunol. 1999 Sep;17(3):219-28 PMID: 10697262
  3. Search for Pneumocystis carinii DNA in upper and lower respiratory tract of humans.
    Diagn Microbiol Infect Dis. 2000 Jul;37(3):161-4 PMID: 10904188
  4. Pathogenic mechanisms underlying the clinical symptoms of allergic rhinitis.
    Am J Rhinol. 2000 Sep-Oct;14(5):325-33 PMID: 11068658
  5. Collection of mucosal secretion by synthetic discs for quantitation of secretory IgA and bacteria.
    J Immunol Methods. 1984 Oct 26;73(2):251-7 PMID: 6436383
  6. Amino acid composition of human nasal mucus. Normal subjects.
    Ann Otol Rhinol Laryngol. 1973 Jan-Feb;82(1):75-9 PMID: 4685573
  7. [Nasal soluble levels of ICAM-1 in allergic rhinitis].
    Ann Otolaryngol Chir Cervicofac. 2000 Mar;117(2):91-7 PMID: 10739998
  8. Levels of intracellular protein and messenger RNA of mucin and lysozyme in normal human nasal and polyp epithelium.
    Laryngoscope. 2000 Feb;110(2 Pt 1):276-80 PMID: 10680929
  9. Lactoferrin and eosinophilic cationic protein in nasal secretions of patients with experimental rhinovirus colds, natural colds, and presumed acute community-acquired bacterial sinusitis.
    J Clin Microbiol. 2000 Aug;38(8):3100-2 PMID: 10921988
  10. A new collection method for the evaluation of nasal mucus proteins.
    Clin Exp Allergy. 1998 Jul;28(7):881-8 PMID: 9720823
  11. [Methods for monitoring of therapeutic efficacy in immunotherapy of allergic rhinitis].
    Wien Med Wochenschr. 1999;149(14-15):394-402 PMID: 10584282
  12. Electrophoretic pattern of physiological human nasal secretions.
    Rhinology. 1996 Sep;34(3):147-50 PMID: 8938882
  13. Secretion of TGF-betal, TGF-beta2, EGF and PDGF into nasal fluid after sinus surgery.
    Eur Arch Otorhinolaryngol. 2002 May;259(5):234-8 PMID: 12107524
  14. Nasal secretions from patients with polyps and healthy individuals, collected with a new aspiration system: evaluation of total protein and immunoglobulin concentrations.
    Ann Clin Biochem. 1991 May;28 ( Pt 3):260-6 PMID: 1872572
  15. Physiologic responses and histamine release after nasal antigen challenge. Effect of atropine.
    Am J Respir Crit Care Med. 1994 Jun;149(6):1457-65 PMID: 7516250
  16. Immunoglobulins in nasal secretions of healthy humans: structural integrity of secretory immunoglobulin A1 (IgA1) and occurrence of neutralizing antibodies to IgA1 proteases of nasal bacteria.
    Clin Diagn Lab Immunol. 2000 Jan;7(1):31-9 PMID: 10618273
  17. Morphologic features of airway surface epithelial cells and glands.
    Am Rev Respir Dis. 1983 Aug;128(2 Pt 2):S14-20 PMID: 6881701
  18. [Use of a rapid detection assay for influenza virus, on nasal aspirate specimens].
    Kansenshogaku Zasshi. 1999 Oct;73(10):1064-8 PMID: 10565123
  19. Simultaneous reversed-phase extraction of lipoxygenase and cyclooxygenase metabolites of arachidonic acid in nasal secretions: methodological aspects.
    J Chromatogr. 1990 Nov 16;532(2):217-25 PMID: 1964689
  20. Chemokines in nasal secretions of normal adults experimentally infected with respiratory syncytial virus.
    Clin Immunol. 2000 Oct;97(1):43-9 PMID: 10998316
  21. A simple and reproducible method for collecting nasal secretions in frail elderly adults, for measurement of virus-specific IgA.
    J Infect Dis. 1999 May;179(5):1268-73 PMID: 10191235
  22. Elevated levels of myeloperoxidase, pro-inflammatory cytokines and chemokines in naturally acquired upper respiratory tract infections.
    Eur Arch Otorhinolaryngol. 2001 Oct;258(8):406-12 PMID: 11724263
  23. [Quantitative cytology of nasal secretions collected by lavage and blowing. Nasal cytometry].
    Ann Otolaryngol Chir Cervicofac. 1992;109(1):39-46 PMID: 1575417
  24. [Usefulness of the urea method in nasal discharge analysis].
    Nihon Jibiinkoka Gakkai Kaiho. 2000 Jun;103(6):734-41 PMID: 10897585
  25. Nasal biopsy is superior to nasal smear for finding eosinophils in nonallergic rhinitis.
    Allergy. 1997 Mar;52(3):338-41 PMID: 9140527
Article Info
Journal
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Abbr.
Eur Arch Otorhinolaryngol
ISSN
0937-4477
DOI
10.1007/s00405-003-0691-y
Email
[email protected]
Published
2004-05-00
Epub
2003-00-09
Pages
242-6
Language
English
Region
Germany
NLM ID
9002937
Subset
IM
External Links
PubMed source DOI: 10.1007/s00405-003-0691-y View journal details
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