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PMID: 14614626 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PCC1: a merging point for pathogen defence and circadian signalling in Arabidopsis.

Planta ·Vol. 218 ·No. 4 ·2004-02-00 ·Pages 552-61

Sauerbrunn N, Schlaich NL

Abstract

Using a cDNA-array we identified expressed sequence tag 163B24T7 as rapidly up-regulated in Arabidopsis thaliana (L.) Heynh. after pathogen exposure. Detailed expression analysis revealed that the corresponding gene is up-regulated not only after exposure to avirulent Pseudomonas syringae pv. tomato but also to virulent strains. This up-regulation is dependent on functional salicylic acid defence-signalling pathways. Moreover, we found the gene was circadian-regulated, showing peaks of expression at the end of the day. Using plants overexpressing the clock component CCA1, we showed that the PCC1 gene is regulated by the inner clock of Arabidopsis. Accordingly, we named the gene PCC1, for pathogen and circadian controlled. PCC1 is a member of a novel family of six small polypeptides in Arabidopsis. A functional role for PCC1 in plant defence was demonstrated since plants overexpressing PCC1 are resistant against normally virulent oomycetes. Thus, PCC1 demonstrates a potential interrelationship between pathogen and circadian signalling pathways.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,physiology Arabidopsis Proteins/genetics,physiology Base Sequence Circadian Rhythm/physiology Cloning, Molecular Conserved Sequence DNA Primers Gene Expression Regulation, Plant Molecular Sequence Data Plant Diseases/microbiology Pseudomonas syringae/pathogenicity RNA, Messenger/genetics Sequence Alignment Sequence Homology, Amino Acid Signal Transduction/physiology Transcription, Genetic
Chemicals
Arabidopsis Proteins DNA Primers PCC1 protein, Arabidopsis RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sauerbrunn Nicolas
Institut Bio III (Pflanzenphysiologie), RWTH Aachen, 52056 Aachen, Germany.
Schlaich Nikolaus L
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-003-1143-z
Published
2004-02-00
Epub
2003-00-12
Pages
552-61
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-003-1143-z View journal details
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