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PMID: 14769862 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bicarbonate-responsive "soluble" adenylyl cyclase defines a nuclear cAMP microdomain.

The Journal of cell biology ·Vol. 164 ·No. 4 ·2004-02-16 ·Pages 527-34

Zippin JH, Farrell J, Huron D, Kamenetsky M, Hess KC, Fischman DA, Levin LR, Buck J

Abstract

Bicarbonate-responsive "soluble" adenylyl cyclase resides, in part, inside the mammalian cell nucleus where it stimulates the activity of nuclear protein kinase A to phosphorylate the cAMP response element binding protein (CREB). The existence of this complete and functional, nuclear-localized cAMP pathway establishes that cAMP signals in intracellular microdomains and identifies an alternate pathway leading to CREB activation.

MeSH Terms
Adenylyl Cyclase Inhibitors Adenylyl Cyclases/metabolism Animals Bicarbonates/metabolism Cell Line Cell Nucleus/enzymology Chlorocebus aethiops Cyclic AMP/metabolism Cyclic AMP Response Element-Binding Protein/metabolism Cyclic AMP-Dependent Protein Kinases/metabolism Enzyme Activation Gene Expression Regulation Hepatocytes/cytology,metabolism Humans Immunohistochemistry Phosphorylation Rats Second Messenger Systems/physiology
Chemicals
Adenylyl Cyclase Inhibitors Bicarbonates Cyclic AMP Response Element-Binding Protein Cyclic AMP Cyclic AMP-Dependent Protein Kinases Adenylyl Cyclases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zippin Jonathan H
Department of Pharmacology, Joan and Sanford I. Weill Medical College and Graduate School of Medical Sciences of Cornell University, 1300 York Ave., New York, NY 10021, USA.
Farrell Jeanne
Huron David
Kamenetsky Margarita
Hess Kenneth C
Fischman Donald A
Levin Lonny R
Buck Jochen
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200311119
Published
2004-02-16
Epub
2004-00-09
Pages
527-34
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172001
Subset
IM
Grants
NIGMS NIH HHS · GM07739 · United States
NIAMS NIH HHS · R01 AR032147 · United States
NICHD NIH HHS · HD38722 · United States
NICHD NIH HHS · R01 HD038722 · United States
NIGMS NIH HHS · R01 GM062328 · United States
NIAMS NIH HHS · AR32147 · United States
NICHD NIH HHS · HD42060 · United States
NIGMS NIH HHS · T32 GM007739 · United States
NIGMS NIH HHS · GM62328 · United States
External Links
PubMed source DOI: 10.1083/jcb.200311119 PMC Full Text View journal details
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