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PMID: 14960714 Published · epublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1.

Nucleic acids research ·Vol. 32 ·No. 3 ·2004-00-00 ·Pages 977-88

Rasko DA, Ravel J, Økstad OA, Helgason E, Cer RZ, Jiang L, Shores KA, Fouts DE, Tourasse NJ, Angiuoli SV, Kolonay J, Nelson WC, Kolstø AB, Fraser CM, Read TD

Abstract

We sequenced the complete genome of Bacillus cereus ATCC 10987, a non-lethal dairy isolate in the same genetic subgroup as Bacillus anthracis. Comparison of the chromosomes demonstrated that B.cereus ATCC 10987 was more similar to B.anthracis Ames than B.cereus ATCC 14579, while containing a number of unique metabolic capabilities such as urease and xylose utilization and lacking the ability to utilize nitrate and nitrite. Additionally, genetic mechanisms for variation of capsule carbohydrate and flagella surface structures were identified. Bacillus cereus ATCC 10987 contains a single large plasmid (pBc10987), of approximately 208 kb, that is similar in gene content and organization to B.anthracis pXO1 but is lacking the pathogenicity-associated island containing the anthrax lethal and edema toxin complex genes. The chromosomal similarity of B.cereus ATCC 10987 to B.anthracis Ames, as well as the fact that it contains a large pXO1-like plasmid, may make it a possible model for studying B.anthracis plasmid biology and regulatory cross-talk.

MeSH Terms
Adaptation, Physiological Antigens, Bacterial/biosynthesis,genetics Bacillus anthracis/classification,genetics Bacillus cereus/classification,genetics,metabolism Bacterial Capsules/immunology Base Sequence Chromosomes, Bacterial Conserved Sequence Flagella/immunology Genome, Bacterial Phylogeny Plasmids/chemistry,classification,genetics Recombination, Genetic Regulon
Chemicals
Antigens, Bacterial
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Rasko David A
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
Ravel Jacques
Økstad Ole Andreas
Helgason Erlendur
Cer Regina Z
Jiang Lingxia
Shores Kelly A
Fouts Derrick E
Tourasse Nicolas J
Angiuoli Samuel V
Kolonay James
Nelson William C
Kolstø Anne-Brit
Fraser Claire M
Read Timothy D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkh258
Published
2004-00-00
Epub
2004-00-11
Pages
977-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC373394
Subset
IM
Grants
NIAID NIH HHS · N01AI15447 · United States
External Links
PubMed source DOI: 10.1093/nar/gkh258 PMC Full Text View journal details
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