Home LiteratureArticle Details
PMID: 1505513 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel and conserved salt-induced protein is an important determinant of salt tolerance in yeast.

The EMBO journal ·Vol. 11 ·No. 9 ·1992-09-00 ·Pages 3157-64

Gaxiola R, de Larrinoa IF, Villalba JM, Serrano R

Abstract

We have isolated a novel yeast gene, HAL1, which upon overexpression improves growth under salt stress. In addition, disruption of this gene decreases salt tolerance. Therefore HAL1 constitutes a rate-limiting determinant for halotolerance. It encodes a polar protein of 32 kDa located in the yeast cytoplasm and unrelated to sequences in data banks. The expression of this gene is increased by high concentrations of either NaCl, KCl or sorbitol. On the other hand, the growth advantage obtained by overexpression of HAL1 is specific for NaCl stress. In cells overexpressing HAL1, sodium toxicity seems to be counteracted by an increased accumulation of potassium. The HAL1 protein could interact with the transport systems which determine intracellular K+ homeostasis. The HAL1 gene and encoded protein are conserved in plants, being induced in these organisms by salt stress and abscisic acid. These results suggest that yeast serves as a convenient model system for the molecular biology of plant salt tolerance.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Cell Division/drug effects Cloning, Molecular Cytoplasm/metabolism DNA, Fungal Fungal Proteins/biosynthesis,genetics Gene Expression Regulation, Fungal Genes, Fungal Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutagenesis Plants/drug effects,genetics Potassium Chloride/pharmacology RNA, Messenger/genetics Saccharomyces cerevisiae/drug effects,genetics,physiology Saccharomyces cerevisiae Proteins Salts/pharmacology Sequence Homology, Nucleic Acid Sodium Chloride/pharmacology Sorbitol/pharmacology
Chemicals
DNA, Fungal Fungal Proteins HAL1 protein, S cerevisiae Intracellular Signaling Peptides and Proteins RNA, Messenger Saccharomyces cerevisiae Proteins Salts Sodium Chloride Sorbitol Potassium Chloride
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gaxiola R
European Molecular Biology Laboratory, Heidelberg, Germany.
de Larrinoa I F
Villalba J M
Serrano R
References (50)
50 references, click to expand
  1. Identification of the viroid-induced tomato pathogenesis-related (PR) protein P23 as the thaumatin-like tomato protein NP24 associated with osmotic stress.
    Plant Mol Biol. 1991 May;16(5):931-4 PMID: 1859873
  2. Artifactual immunofluorescent labelling in yeast, demonstrated by affinity purification of antibody.
    Yeast. 1987 Jun;3(2):63-70 PMID: 3332966
  3. Putting the HO gene to work: practical uses for mating-type switching.
    Methods Enzymol. 1991;194:132-46 PMID: 2005783
  4. Micromanipulation and dissection of asci.
    Methods Enzymol. 1991;194:21-37 PMID: 2005789
  5. Immunofluorescence methods for yeast.
    Methods Enzymol. 1991;194:565-602 PMID: 2005809
  6. A plant leucine zipper protein that recognizes an abscisic acid response element.
    Science. 1990 Oct 12;250(4978):267-71 PMID: 2145628
  7. Characterization of a rice gene showing organ-specific expression in response to salt stress and drought.
    Plant Cell. 1990 Jan;2(1):19-27 PMID: 2152105
  8. Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation.
    Plant Cell. 1990 Jul;2(7):673-84 PMID: 2152343
  9. TRK2 is required for low affinity K+ transport in Saccharomyces cerevisiae.
    Genetics. 1990 Jun;125(2):305-12 PMID: 2199312
  10. Single-step purification of shuttle vectors from yeast for high frequency back-transformation into E. coli.
    Nucleic Acids Res. 1990 Sep 11;18(17):5319 PMID: 2205843
  11. Regulation of potassium fluxes in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1990 Nov 16;1029(2):211-7 PMID: 2245208
  12. Physiology of mutants with reduced expression of plasma membrane H+-ATPase.
    Yeast. 1989 Jul-Aug;5(4):307-19 PMID: 2528864
  13. ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7866-70 PMID: 2530577
  14. Arabidopsis, a useful weed.
    Cell. 1989 Jan 27;56(2):263-9 PMID: 2643481
  15. Physiological and genetic responses of bacteria to osmotic stress.
    Microbiol Rev. 1989 Mar;53(1):121-47 PMID: 2651863
  16. H+-ATPase from plasma membranes of Saccharomyces cerevisiae and Avena sativa roots: purification and reconstitution.
    Methods Enzymol. 1988;157:533-44 PMID: 2906717
  17. Tight control of the amount of yeast plasma membrane ATPase during changes in growth conditions and gene dosage.
    FEBS Lett. 1987 Nov 16;224(1):193-7 PMID: 2960559
  18. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
    Nucleic Acids Res. 1988 Aug 11;16(15):7583-600 PMID: 2970625
  19. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  21. Eviction and transplacement of mutant genes in yeast.
    Methods Enzymol. 1983;101:211-28 PMID: 6310325
  22. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.
    Anal Biochem. 1983 Jul 1;132(1):14-9 PMID: 6312834
  23. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  24. Targeted selection of recombinant clones through gene dosage effects.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6750-4 PMID: 6316322
  25. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  26. The new yeast genetics.
    Nature. 1983 Sep 29-Oct 5;305(5933):391-7 PMID: 6353245
  27. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
    Anal Biochem. 1984 Jan;136(1):175-9 PMID: 6424501
  28. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  29. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  30. The molecular basis of dominance.
    Genetics. 1981 Mar-Apr;97(3-4):639-66 PMID: 7297851
  31. On the evolution of the adaptation of Lophopyrum elongatum to growth in saline environments.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3805-9 PMID: 16593932
  32. Regulation of potassium absorption in barley roots: an allosteric model.
    Plant Physiol. 1976 Jul;58(1):33-7 PMID: 16659615
  33. Comparative studies on the sodium, potassium, and chloride relations of a wild halophytic and a domestic salt-sensitive tomato species.
    Plant Physiol. 1981 Dec;68(6):1308-13 PMID: 16662098
  34. Abscisic Acid accelerates adaptation of cultured tobacco cells to salt.
    Plant Physiol. 1985 Sep;79(1):138-42 PMID: 16664358
  35. Studies on H-Translocating ATPases in Plants of Varying Resistance to Salinity : I. Salinity during Growth Modulates the Proton Pump in the Halophyte Atriplex nummularia.
    Plant Physiol. 1986 Aug;81(4):1050-6 PMID: 16664942
  36. Salt tolerance in suspension cultures of sugar beet : induction of na/h antiport activity at the tonoplast by growth in salt.
    Plant Physiol. 1987 Apr;83(4):884-7 PMID: 16665356
  37. NaCl Induces a Na/H Antiport in Tonoplast Vesicles from Barley Roots.
    Plant Physiol. 1988 Jan;86(1):231-6 PMID: 16665872
  38. Molecular Cloning of Osmotin and Regulation of Its Expression by ABA and Adaptation to Low Water Potential.
    Plant Physiol. 1989 Jul;90(3):1096-101 PMID: 16666857
  39. Enhanced H Transport Capacity and ATP Hydrolysis Activity of the Tonoplast H-ATPase after NaCl Adaptation.
    Plant Physiol. 1990 Oct;94(2):524-30 PMID: 16667744
  40. Characterization of Five Abscisic Acid-Responsive cDNA Clones Isolated from the Desiccation-Tolerant Plant Craterostigma plantagineum and Their Relationship to Other Water-Stress Genes.
    Plant Physiol. 1990 Dec;94(4):1682-8 PMID: 16667902
  41. Enhanced Net K Uptake Capacity of NaCl-Adapted Cells.
    Plant Physiol. 1991 Apr;95(4):1265-9 PMID: 16668122
  42. Saline culture of crops: a genetic approach.
    Science. 1980 Oct 24;210(4468):399-404 PMID: 17837407
  43. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  44. Abscisic acid and water-stress induce the expression of a novel rice gene.
    EMBO J. 1988 Aug;7(8):2279-86 PMID: 2973410
  45. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  46. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  47. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions.
    Gene. 1986;45(3):299-310 PMID: 3026915
  48. TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jul;8(7):2848-59 PMID: 3043197
  49. pUEX, a bacterial expression vector related to pEX with universal host specificity.
    Nucleic Acids Res. 1987 Dec 10;15(23):10056 PMID: 3320953
  50. Osmoregulation in Saccharomyces cerevisiae. Studies on the osmotic induction of glycerol production and glycerol-3-phosphate dehydrogenase (NAD+)
    FEBS Lett. 1991 Jul 29;286(1-2):13-7 PMID: 1864360
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-09-00
Pages
3157-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556849
Subset
IM
Databases
GENBANK
X66121, X66358, X66359, X66360, X66361, X66362, X66363, X66364, X66365, X67559
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2