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PMID: 15065656 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Interplay between DNA replication, recombination and repair based on the structure of RecG helicase.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences ·Vol. 359 ·No. 1441 ·2004-01-29 ·Pages 49-59

Briggs GS, Mahdi AA, Weller GR, Wen Q, Lloyd RG

Abstract

Recent studies in Escherichia coli indicate that the interconversion of DNA replication fork and Holliday junction structures underpins chromosome duplication and helps secure faithful transmission of the genome from one generation to the next. It facilitates interplay between DNA replication, recombination and repair, and provides means to rescue replication forks stalled by lesions in or on the template DNA. Insight into how this interconversion may be catalysed has emerged from genetic, biochemical and structural studies of RecG protein, a member of superfamily 2 of DNA and RNA helicases. We describe how a single molecule of RecG might target a branched DNA structure and translocate a single duplex arm to drive branch migration of a Holliday junction, interconvert replication fork and Holliday junction structures and displace the invading strand from a D loop formed during recombination at a DNA end. We present genetic evidence suggesting how the latter activity may provide an efficient pathway for the repair of DNA double-strand breaks that avoids crossing over, thus facilitating chromosome segregation at cell division.

MeSH Terms
Chromosome Segregation/physiology DNA/metabolism DNA Helicases/chemistry,metabolism DNA Repair/physiology DNA Replication/physiology DNA, Cruciform/metabolism Escherichia coli Escherichia coli Proteins/chemistry,metabolism Recombination, Genetic/physiology
Chemicals
DNA, Cruciform Escherichia coli Proteins RecG protein, E coli DNA DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Briggs Geoffrey S
Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK.
Mahdi Akeel A
Weller Geoffrey R
Wen Qin
Lloyd Robert G
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Article Info
Journal
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
Abbr.
Philos Trans R Soc Lond B Biol Sci
ISSN
0962-8436
DOI
10.1098/rstb.2003.1364
Published
2004-01-29
Pages
49-59
Language
English
Region
England
NLM ID
7503623
PMCID
PMC1693295
Subset
IM
External Links
PubMed source DOI: 10.1098/rstb.2003.1364 PMC Full Text View journal details
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