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PMID: 7628432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Branch migration of three-strand recombination intermediates by RecG, a possible pathway for securing exchanges initiated by 3'-tailed duplex DNA.

The EMBO journal ·Vol. 14 ·No. 14 ·1995-07-17 ·Pages 3302-10

Whitby MC, Lloyd RG

Abstract

RecG protein is required for normal levels of recombination and DNA repair in Escherichia coli. This 76 kDa polypeptide is a junction-specific DNA helicase that acts post-synaptically to drive branch migration of Holliday junction intermediates made by RecA during the strand exchange stage of recombination. To gain further insight into the role of RecG, we studied its activity on three-strand intermediates formed by RecA between circular single-stranded and linear duplex DNAs. Once RecA is removed, RecG drives branch migration of these intermediates by a junction-targeted activity that depends on hydrolysis of ATP. RuvAB has a similar activity. However, when RecG is added to a RecA strand exchange reaction it severely reduces the accumulation of joint molecule intermediates by driving branch migration of junctions in the reverse direction to that catalysed by RecA strand exchange. In comparison, RuvAB has little effect on the reaction. We discuss how reverse branch migration by RecG, which acts counter of the 5'-->3' polarity of RecA binding and strand exchange, could serve to promote or abort the early stages of recombination, depending on the orientation of the single DNA strand initiating the exchange relative to the adjacent duplex region.

MeSH Terms
Bacterial Proteins/metabolism Bacteriophage phi X 174/genetics,metabolism Base Sequence DNA Helicases/metabolism DNA Repair DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics Escherichia coli Proteins Models, Genetic Molecular Sequence Data Nucleic Acid Heteroduplexes Rec A Recombinases/metabolism Recombination, Genetic
Chemicals
Bacterial Proteins DNA, Viral DNA-Binding Proteins Escherichia coli Proteins Nucleic Acid Heteroduplexes RuvB protein, Bacteria RecG protein, E coli Rec A Recombinases Holliday junction DNA helicase, E coli DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Whitby M C
Department of Genetics, University of Nottingham, Queens Medical Centre, UK.
Lloyd R G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-07-17
Pages
3302-10
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394398
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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