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PMID: 1508216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pax-8, a paired domain-containing protein, binds to a sequence overlapping the recognition site of a homeodomain and activates transcription from two thyroid-specific promoters.

Molecular and cellular biology ·Vol. 12 ·No. 9 ·1992-09-00 ·Pages 4230-41

Zannini M, Francis-Lang H, Plachov D, Di Lauro R

Abstract

The Pax-8 gene, a member of the murine family of paired box-containing genes (Pax genes), is expressed in adult thyroid and in cultured thyroid cell lines. The Pax-8 protein binds, through its paired domain, to the promoters of thyroglobulin and thyroperoxidase, genes that are exclusively expressed in the thyroid. In both promoters, the binding site of Pax-8 overlaps with that of TTF-1, a homeodomain-containing protein involved in the activation of thyroid-specific transcription. Pax-8 activates transcription from cotransfected thyroperoxidase and thyroglobulin promoters, indicating that it may be involved in the establishment, control, or maintenance of the thyroid-differentiated phenotype. Thus, the promoters of thyroglobulin and thyroperoxidase represent the first identified natural targets for transcriptional activation by a paired domain-containing protein.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Northern DNA HeLa Cells Humans Molecular Sequence Data Peroxidases/genetics Promoter Regions, Genetic Rats Thyroglobulin/genetics Thyroid Gland/enzymology,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Transcription Factors DNA Thyroglobulin Peroxidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zannini M
European Molecular Biology Laboratory, Heidelberg, Germany.
Francis-Lang H
Plachov D
Di Lauro R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.9.4230-4241.1992
Published
1992-09-00
Pages
4230-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360331
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.12.9.4230-4241.1992 PMC Full Text View journal details
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