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PMID: 15131188 Published · ppublish English Journal Article

Systematic 16S rRNA gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans.

Journal of clinical microbiology ·Vol. 42 ·No. 5 ·2004-05-00 ·Pages 2197-202

Drancourt M, Berger P, Raoult D

Abstract

Clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16S rRNA gene sequence analysis. Each isolate yielded a > or =1,400-bp sequence containing <5 ambiguities which was compared with the GenBank 16S rRNA gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (< or =10 cases reported in the literature) human pathogens. Eleven new species, "Actinobaculum massiliae," "Candidatus Actinobaculum timonae," Paenibacillus sanguinis, "Candidatus Bacteroides massiliae," Chryseobacterium massiliae, "Candidatus Chryseobacterium timonae," Paenibacillus massiliensis, "Candidatus Peptostreptococcus massiliae," "Candidatus Prevotella massiliensis," Rhodobacter massiliensis, and "Candidatus Veillonella atypica" were identified. Sixteen species were obtained from humans for the first time. Our results show the important role that 16S rRNA gene sequence-based bacterial identification currently plays in recognizing unusual and emerging bacterial diseases.

MeSH Terms
Adolescent Adult Aged Aged, 80 and over Bacteria/classification,genetics,isolation & purification Bacteria, Anaerobic/classification,genetics,isolation & purification Bacterial Infections/microbiology Bacterial Typing Techniques Child, Preschool Female Genes, Bacterial Gram-Negative Bacteria/classification,genetics,isolation & purification Gram-Positive Cocci/classification,genetics,isolation & purification Gram-Positive Rods/classification,genetics,isolation & purification Humans Infant, Newborn Male Middle Aged RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Species Specificity
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Drancourt M
Assistance Publique-Hopitaux de Marseille, Timone, Fédération de Microbiologie Clinique, et Unité des Rickettsies, CNRS UMR 6020, IFR48, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
Berger P
Raoult D
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.42.5.2197-2202.2004
Published
2004-05-00
Pages
2197-202
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC404640
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.42.5.2197-2202.2004 PMC Full Text View journal details
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