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PMID: 1518813 Published · ppublish English Journal Article

Cationic lipids direct a viral glycoprotein into the class I major histocompatibility complex antigen-presentation pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 17 ·1992-09-01 ·Pages 7915-8

Walker C, Selby M, Erickson A, Cataldo D, Valensi JP, Van Nest GV

Abstract

Recombinant glycoprotein B (gB) of herpes simplex virus (HSV) was processed and presented by class I major histocompatibility complex (MHC) molecules after delivery into cells by using N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl sulfate (DOTAP), a commercially available cationic lipid used for DNA transfection. Cells treated with DOTAP-associated gB were susceptible to lysis by class I MHC-restricted, HSV-specific cytotoxic T lymphocytes (CTL), and the treated cells restimulated memory gB-specific CTL activity in spleen cells from HSV-infected mice. gB-specific CTL responses were detected in mice immunized with recombinant gB and DOTAP but not in those receiving gB emulsified in complete Freund's adjuvant. Thus, cationic lipids may facilitate induction of CD8+ T-cell responses in vaccinations with recombinant antigens, and they may serve as readily available reagents for dissecting class I MHC immunity to viruses and other intracellular pathogens.

MeSH Terms
Adjuvants, Immunologic Animals Antigen-Presenting Cells/metabolism CD8 Antigens/analysis Cytotoxicity, Immunologic Fatty Acids, Monounsaturated/pharmacology Histocompatibility Antigens Class I/metabolism Immunologic Memory Mice Mice, Inbred C57BL Peptides/immunology Quaternary Ammonium Compounds/pharmacology T-Lymphocytes, Cytotoxic/immunology Vaccines, Synthetic/immunology Viral Envelope Proteins/immunology
Chemicals
Adjuvants, Immunologic CD8 Antigens Fatty Acids, Monounsaturated Histocompatibility Antigens Class I Peptides Quaternary Ammonium Compounds Vaccines, Synthetic Viral Envelope Proteins glycoprotein B, Simplexvirus 1,2-dioleoyloxy-3-(trimethylammonium)propane
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Walker C
Chiron Corporation, Emeryville, CA 94608.
Selby M
Erickson A
Cataldo D
Valensi J P
Van Nest G V
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.17.7915
Published
1992-09-01
Pages
7915-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49825
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.17.7915 PMC Full Text View journal details
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