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PMID: 1522214 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex.

The Journal of clinical investigation ·Vol. 90 ·No. 3 ·1992-09-00 ·Pages 1061-6

Steiner G, Hartmuth K, Skriner K, Maurer-Fogy I, Sinski A, Thalmann E, Hassfeld W, Barta A, Smolen JS

Abstract

RA33 is a nuclear autoantigen with an apparent molecular mass of 33 kD. Autoantibodies against RA33 are found in about 30% of sera from RA patients, but only occasionally in sera from patients with other connective tissue diseases. To characterize RA33, the antigen was purified from HeLa cell nuclear extracts to more than 90% homogeneity by affinity chromatography on heparin-Sepharose and by chromatofocusing. Sequence analysis of five tryptic peptides revealed that their sequences matched corresponding sequences of the A2 protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Furthermore, RA33 was shown to be present in the 40S hnRNP complex and to behave indistinguishably from A2 in binding to single stranded DNA. In summary, these data strongly indicate that RA33 and A2 are the same protein, and thus identify on a molecular level a new autoantigen.

MeSH Terms

Amino Acid Sequence Antigens, Nuclear Arthritis, Rheumatoid/immunology Autoantigens/analysis,immunology,isolation & purification DNA, Single-Stranded/metabolism HeLa Cells Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Sequence Data Nuclear Proteins/analysis,immunology,isolation & purification Ribonucleoproteins/analysis

Chemicals

Antigens, Nuclear Autoantigens DNA, Single-Stranded Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Nuclear Proteins Ribonucleoproteins hnRNP A2

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Steiner G

Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, Austria.

Hartmuth K

Skriner K

Maurer-Fogy I

Sinski A

Thalmann E

Hassfeld W

Barta A

Smolen J S

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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI115921

Published

1992-09-00

Pages

1061-6

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC329965

Subset

External Links

PubMed source DOI: 10.1172/JCI115921 PMC Full Text View journal details

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Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex.

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