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PMID: 1691095 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alternative splicing in the human gene for the core protein A1 generates another hnRNP protein.

The EMBO journal ·Vol. 9 ·No. 4 ·1990-04-00 ·Pages 1229-35

Buvoli M, Cobianchi F, Bestagno MG, Mangiarotti A, Bassi MT, Biamonti G, Riva S

Abstract

The human hnRNP core protein A1 (34 kd) is encoded by a 4.6 kb gene split into 10 exons. Here we show that the A1 gene can be differentially spliced by the addition of an extra exon. The new transcript encodes a minor protein of the hnRNP complex, here defined A1B protein, with a calculated mol. wt of 38 kd, that coincides with a protein previously designated as B2 by some authors. In vitro translation of the mRNAs selected by hybridization with A1 cDNA produced two proteins of 34 and 38 kd; Northern blot analysis of poly(A)+ RNA from HeLa cells revealed that the abundance of the A1B mRNA was approximately 5% that of A1. The A1B protein was detected by Western blotting with an anti-A1 monoclonal antibody both in enriched preparations of basic hnRNP proteins and in 40S hnRNP particles. The A1B protein exhibits a significantly higher affinity than A1 for ssDNA. The recombinant A1B protein, expressed in Escherichia coli, shows the same electrophoretic mobility and charge as the cellular one.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Western Cloning, Molecular DNA, Neoplasm/genetics Escherichia coli/genetics Exons Genes HeLa Cells/metabolism Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Sequence Data Oligonucleotide Probes Poly A/genetics,isolation & purification Protein Biosynthesis RNA/genetics,isolation & purification RNA Splicing RNA, Heterogeneous Nuclear/genetics RNA, Messenger Ribonucleoproteins/genetics Transcription, Genetic
Chemicals
DNA, Neoplasm Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Oligonucleotide Probes RNA, Heterogeneous Nuclear RNA, Messenger Ribonucleoproteins Poly A RNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Buvoli M
Istituto di Genetica Biochimica ed Evoluzionistica CNR, Pavia, Italy.
Cobianchi F
Bestagno M G
Mangiarotti A
Bassi M T
Biamonti G
Riva S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-04-00
Pages
1229-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551799
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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