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PMID: 15229284 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans.

Molecular biology of the cell ·Vol. 15 ·No. 9 ·2004-09-00 ·Pages 4179-90

Smith DA, Nicholls S, Morgan BA, Brown AJ, Quinn J

Abstract

Previous work has implicated the Hog1 stress-activated protein kinase (SAPK) in osmotic and oxidative stress responses in the human pathogen Candida albicans. In this study, we have characterized the role of Hog1 in mediating these and other stress responses in C. albicans. We provide evidence that a SAPK-dependent core stress response exists in this pathogen. The Hog1 SAPK is phosphorylated and it accumulates in the nucleus in response to diverse stress conditions. In addition, we have identified Hog1-regulated genes that are induced in response to stress conditions that activate Hog1. These analyses reveal both activator and repressor functions for the Hog1 SAPK. Our results also demonstrate that stress cross-protection, a classical hallmark of the core stress response, occurs in C. albicans between stresses that activate the Hog1 SAPK. Importantly, we find that the core stress response in C. albicans has adapted to the environmental niche of this human pathogen. This niche specificity is reflected by the specific environmental conditions that drive the Hog1-regulated core stress response in C. albicans and by differences in the molecular circuitry that control this response.

MeSH Terms
Base Sequence Candida albicans/enzymology,genetics,pathogenicity Cell Nucleus/enzymology DNA, Fungal/genetics Enzyme Activation Gene Expression Regulation, Fungal Genes, Fungal Genetic Complementation Test Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinases/genetics,metabolism Models, Biological Molecular Sequence Data Osmotic Pressure Oxidative Stress Phenotype Phosphorylation Saccharomyces cerevisiae/enzymology,genetics Schizosaccharomyces/enzymology,genetics Schizosaccharomyces pombe Proteins/genetics,metabolism Species Specificity
Chemicals
DNA, Fungal Schizosaccharomyces pombe Proteins Mitogen-Activated Protein Kinases sty1 protein, S pombe
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Smith Deborah A
School of Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom.
Nicholls Susan
Morgan Brian A
Brown Alistair J P
Quinn Janet
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-03-0181
Published
2004-09-00
Epub
2004-00-30
Pages
4179-90
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC515350
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/C510391/1 · United Kingdom
External Links
PubMed source DOI: 10.1091/mbc.e04-03-0181 PMC Full Text View journal details
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