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PMID: 15280509 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

GATA-1 and NF-Y cooperate to mediate erythroid-specific transcription of Gfi-1B gene.

Nucleic acids research ·Vol. 32 ·No. 13 ·2004-00-00 ·Pages 3935-46

Huang DY, Kuo YY, Lai JS, Suzuki Y, Sugano S, Chang ZF

Abstract

Expression of Gfi (growth factor-independence)-1B, a Gfi-1-related transcriptional repressor, is restricted to erythroid lineage cells and is essential for erythropoiesis. We have determined the transcription start site of the human Gfi-1B gene and located its first non-coding exon approximately 7.82 kb upstream of the first coding exon. The genomic sequence preceding this first non-coding exon has been identified to be its erythroid-specific promoter region in K562 cells. Using gel-shift and chromatin immunoprecipitation (ChIP) assays, we have demonstrated that NF-Y and GATA-1 directly participate in transcriptional activation of the Gfi-1B gene in K562 cells. Ectopic expression of GATA-1 markedly stimulates the activity of the Gfi-1B promoter in a non-erythroid cell line U937. Interestingly, our results have indicated that this GATA-1-mediated trans-activation is dependent on NF-Y binding to the CCAAT site. Here we conclude that functional cooperation between GATA-1 and NF-Y contributes to erythroid-specific transcriptional activation of Gfi-1B promoter.

MeSH Terms
5' Untranslated Regions Base Sequence Binding Sites CCAAT-Binding Factor/physiology DNA-Binding Proteins/physiology Erythroid Cells/metabolism Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Humans K562 Cells Molecular Sequence Data Promoter Regions, Genetic Proto-Oncogene Proteins/biosynthesis,genetics Repressor Proteins/biosynthesis,genetics Transcription Factors/physiology Transcriptional Activation U937 Cells
Chemicals
5' Untranslated Regions CCAAT-Binding Factor DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor GATA1 protein, human GFI1B protein, human Proto-Oncogene Proteins Repressor Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Huang Duen-Yi
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, No. 1 Jen Ai Road 1st Section, Taipei, Taiwan, Republic of China.
Kuo Yuan-Yeh
Lai Jiann-Shiun
Suzuki Yutaka
Sugano Sumio
Chang Zee-Fen
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkh719
Published
2004-00-00
Epub
2004-00-27
Pages
3935-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC506805
Subset
IM
Databases
GENBANK
AY428733
External Links
PubMed source DOI: 10.1093/nar/gkh719 PMC Full Text View journal details
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