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PMID: 1406646 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct roles for the two cGATA-1 finger domains.

Molecular and cellular biology ·Vol. 12 ·No. 10 ·1992-10-00 ·Pages 4562-70

Yang HY, Evans T

Abstract

We have generated and analyzed by functional assays mutations of the chicken erythroid transcription factor GATA-1. The cGATA-1 protein contains two related finger domains highly conserved across species and characteristic of the family of GATA-binding factors. We find that mutations in the C-terminal finger or adjacent basic region abolish sequence-specific DNA binding, confirming that this region constitutes a novel DNA-binding domain sufficient to recognize the consensus WGATAR motif. At least three separate regions outside of this finger II domain contribute in a cooperative manner to the trans-activation potential of the protein. As expected from previous results analyzing the mouse homolog, we find that the N-terminal finger plays a role in DNA binding by affecting the stability of the DNA-protein complex. In addition, we find mutations of finger I subtly altered in DNA-binding function which greatly diminish trans-activation. Our results support the notion that the GATA-1 protein must be positioned precisely on the GATA cis element to enable the activation of target genes.

MeSH Terms
Animals Base Sequence Cell Line Chick Embryo DNA/metabolism DNA-Binding Proteins/chemistry,genetics,physiology Erythroid-Specific DNA-Binding Factors Methylation Molecular Sequence Data Mutagenesis, Site-Directed Transcription Factors/chemistry,genetics,physiology Transcriptional Activation Zinc Fingers/genetics,physiology
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Transcription Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yang H Y
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Evans T
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29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.10.4562-4570.1992
Published
1992-10-00
Pages
4562-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360383
Subset
IM
Grants
NIDDK NIH HHS · DK44167 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.10.4562-4570.1992 PMC Full Text View journal details
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