Home LiteratureArticle Details
PMID: 1701019 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulated expression of globin chains and the erythroid transcription factor GATA-1 during erythropoiesis in the developing mouse.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6596-606

Whitelaw E, Tsai SF, Hogben P, Orkin SH

Abstract

Erythropoiesis in vertebrates is characterized by sequential changes in erythropoietic site, erythroblast morphology, and hemoglobin synthesis. We have examined the expression of globin chains and the major erythroid transcription factor GATA-1 (previously known as GF-1/NF-E1/Eryf 1) from days 7.5 to 17.5 of mouse development. mRNAs for embryonic (epsilon y2, beta H1, and zeta) and adult (alpha and beta) globin chains were quantitated by RNase protection assays. Switching of globins within the alpha-globin cluster (alpha and zeta) was not strictly coordinated with that within the beta-globin cluster (epsilon y2, beta H1, and beta). Regulation of globin switches during development was primarily transcriptional. Of particular note, we found two developmental switches (beta H1 to epsilon y2 and epsilon y2 to beta) in the mouse, more analogous than previously thought to shifts found in human development. The erythroid transcription factor GATA-1, believed to be a principal regulator of genes expressed in erythroid cells, first appeared in the embryo in yolk sac at the time of blood island formation and remained at a low level during embryonic erythropoiesis (8 to 11 days) relative to that found later in fetal liver (12 to 15 days). The rise in GATA-1 mRNA in fetal liver paralleled and preceded the rapid accumulation of adult beta-globin RNA. RNase protection assays and a GATA-1-specific peptide antiserum were used to establish that a single GATA-1 polypeptide is expressed throughout mouse development. Overall, these findings suggest that the levels of this erythroid transcription factor during development may contribute to the differential gene activation characteristic of definitive versus primitive erythropoiesis.

MeSH Terms
Animals Crosses, Genetic Embryo, Mammalian Embryonic and Fetal Development Erythropoiesis Gene Expression Regulation Globins/genetics Liver/embryology,metabolism Mice Multigene Family RNA/genetics,isolation & purification Transcriptional Activation
Chemicals
RNA Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Whitelaw E
Sir William Dunn School of Pathology, University of Oxford, England.
Tsai S F
Hogben P
Orkin S H
References (49)
49 references, click to expand
  1. Increased erythroid-specific expression of a mutated HPFH gamma-globin promoter requires the erythroid factor NFE-1.
    Nucleic Acids Res. 1989 Jul 25;17(14):5509-16 PMID: 2474800
  2. Biosynthesis and stability of globin mRNA in cultured erythroleukemic Friend cells.
    Cell. 1976 Aug;8(4):495-503 PMID: 954102
  3. Developmental regulation of beta-globin gene switching.
    Cell. 1988 Oct 7;55(1):17-26 PMID: 3167976
  4. Mutational analysis of the chicken beta-globin enhancer reveals two positive-acting domains.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6267-71 PMID: 3166139
  5. An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5976-80 PMID: 3413070
  6. The beta-globin stage selector element factor is erythroid-specific promoter/enhancer binding protein NF-E4.
    Genes Dev. 1989 Dec;3(12A):1845-59 PMID: 2620825
  7. A 3' enhancer is required for temporal and tissue-specific transcriptional activation of the chicken adult beta-globin gene.
    Nature. 1986 Oct 23-29;323(6090):731-4 PMID: 3022151
  8. Rapid reprogramming of globin gene expression in transient heterokaryons.
    Cell. 1986 Aug 15;46(4):591-602 PMID: 3731277
  9. The "beta-like-globin" gene domain in human erythroid cells.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8 PMID: 3879975
  10. Erythroid-specific expression of human beta-globin genes in transgenic mice.
    EMBO J. 1985 Jul;4(7):1715-23 PMID: 2992937
  11. An enhancer element lies 3' to the human A gamma globin gene.
    EMBO J. 1987 Oct;6(10):2997-3004 PMID: 3691478
  12. A molecular analysis of mouse development from 8 to 10 days post coitum detects changes only in embryonic globin expression.
    Development. 1987 Apr;99(4):493-500 PMID: 2444404
  13. High-level erythroid expression of human alpha-globin genes in transgenic mice.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):37-41 PMID: 2911581
  14. GATAAG; a cis-control region binding an erythroid-specific nuclear factor with a role in globin and non-globin gene expression.
    Nucleic Acids Res. 1989 Jan 11;17(1):73-92 PMID: 2911489
  15. Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene.
    Nucleic Acids Res. 1989 Jan 11;17(1):37-54 PMID: 2911469
  16. Globin gene expression in erythroid human fetal liver cells.
    J Clin Invest. 1989 Mar;83(3):1032-8 PMID: 2921315
  17. The human beta-globin promoter; nuclear protein factors and erythroid specific induction of transcription.
    EMBO J. 1988 Dec 20;7(13):4203-12 PMID: 3243278
  18. Increased gamma-globin expression in a nondeletion HPFH mediated by an erythroid-specific DNA-binding factor.
    Nature. 1989 Mar 30;338(6214):435-8 PMID: 2467208
  19. Position-independent, high-level expression of the human beta-globin gene in transgenic mice.
    Cell. 1987 Dec 24;51(6):975-85 PMID: 3690667
  20. Two 3' sequences direct adult erythroid-specific expression of human beta-globin genes in transgenic mice.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7056-60 PMID: 3478680
  21. Detection of two tissue-specific DNA-binding proteins with affinity for sites in the mouse beta-globin intervening sequence 2.
    Mol Cell Biol. 1988 Jan;8(1):381-92 PMID: 3422099
  22. Haemoglobin switching in human embryos: asynchrony of zeta----alpha and epsilon----gamma-globin switches in primitive and definite erythropoietic lineage.
    Nature. 1985 Jan 17-23;313(5999):235-8 PMID: 2578614
  23. Characterization, expression, and evolution of the mouse embryonic zeta-globin gene.
    Mol Cell Biol. 1985 May;5(5):1025-33 PMID: 4000117
  24. Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6548-52 PMID: 2771941
  25. The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein.
    Genes Dev. 1988 Sep;2(9):1089-100 PMID: 2461328
  26. The gradient morphogen bicoid is a concentration-dependent transcriptional activator.
    Cell. 1989 Jun 30;57(7):1259-73 PMID: 2567637
  27. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  28. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  29. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  30. Cell type-specific protein-DNA interactions in the human zeta-globin upstream promoter region: displacement of Sp1 by the erythroid cell-specific factor NF-E1.
    Mol Cell Biol. 1990 Jan;10(1):282-94 PMID: 2403638
  31. Developmental regulation of human fetal-to-adult globin gene switching in transgenic mice.
    Nature. 1990 Mar 22;344(6264):309-13 PMID: 2314472
  32. Expression of an erythroid transcription factor in megakaryocytic and mast cell lineages.
    Nature. 1990 Mar 29;344(6265):444-7 PMID: 2320112
  33. Human gamma- to beta-globin gene switching in transgenic mice.
    Genes Dev. 1990 Mar;4(3):380-9 PMID: 1692558
  34. A factor binding GATAAG confers tissue specificity on the promoter of the human zeta-globin gene.
    Nucleic Acids Res. 1990 Mar 25;18(6):1339-50 PMID: 2326182
  35. Structure and transcription of the mouse erythropoietin receptor gene.
    Mol Cell Biol. 1990 Jul;10(7):3675-82 PMID: 2162479
  36. Structure and evolution of a human erythroid transcription factor.
    Nature. 1990 Jan 4;343(6253):92-6 PMID: 2104960
  37. The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):668-72 PMID: 2300555
  38. Two mouse early embryonic beta-globin gene sequences. Evolution of the nonadult beta-globins.
    J Biol Chem. 1984 Mar 25;259(6):3739-47 PMID: 6323438
  39. Advances in thalassemia research.
    Blood. 1984 Apr;63(4):738-58 PMID: 6200160
  40. The sequence of a mouse embryonic beta-globin gene. Evolution of the gene and its signal region.
    J Biol Chem. 1982 Jan 25;257(2):1048-52 PMID: 6274852
  41. DNA sequence organization of the beta-globin complex in the BALB/c mouse.
    Cell. 1980 Aug;21(1):159-68 PMID: 6250710
  42. The mouse beta h1 gene codes for the z chain of embryonic hemoglobin.
    J Biol Chem. 1984 Jun 10;259(11):7123-8 PMID: 6327707
  43. Adult hemoglobins are synthesized in murine fetal hepatic erythropoietic cells.
    Blood. 1983 Dec;62(6):1280-8 PMID: 6640111
  44. The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution.
    Cell. 1979 Nov;18(3):875-82 PMID: 519760
  45. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes.
    Cell. 1979 Nov;18(3):865-73 PMID: 519759
  46. Hemoglobin ontogeny during normal mouse fetal development.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2853-7 PMID: 111244
  47. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  48. Globin composition and synthesis of hemoglobins in developing fetal mice erythroid cells.
    Science. 1967 Sep 15;157(3794):1327-9 PMID: 6039000
  49. Purification and characterization of an erythroid cell-specific factor that binds the murine alpha- and beta-globin genes.
    Mol Cell Biol. 1989 Jun;9(6):2606-14 PMID: 2761541
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6596-6606.1990
Published
1990-12-00
Pages
6596-606
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362936
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.12.6596-6606.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2