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PMID: 1528899 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3.

Smeekens SP, Montag AG, Thomas G, Albiges-Rizo C, Carroll R, Benig M, Phillips LA, Martin S, Ohagi S, Gardner P

Abstract

Experiments using recombinant vaccinia viruses expressing rat proinsulin I coinfected into COS-7 cells with recombinant vaccinia virus expressing human furin, human PC2, mouse PC3 (subtilisin-related proprotein convertases 1-3, respectively), or yeast Kex2 indicate that in this system both Kex2 and furin produce mature insulin, whereas PC2 selectively cleaves proinsulin at the C-peptide-A-chain junction. This is a property consistent with its probable identity with the rat insulinoma granule type II proinsulin processing activity as described by Davidson et al. [Davidson, H. W., Rhodes, C. J. & Hutton, J. C. (1988) Nature (London) 333, 93-96]. PC3 generates mature insulin but cleaves preferentially at the proinsulin B-chain-C-peptide junction. This pattern of cleavage by PC3 is similar, but not identical, to that of the highly B-chain-C-peptide junction-selective type I activity as described by Davidson et al., perhaps due to the presence of a P4 arginine residue near the C-peptide-A-chain junction unique to the rat proinsulins. These results along with data presented on the expression of both PC2 and PC3 in islet beta cells strongly support the conclusion that these proteases are involved in the conversion of proinsulin to insulin in vivo.

MeSH Terms
Amino Acid Sequence Animals Furin Humans Immunohistochemistry Insulinoma/enzymology Islets of Langerhans/enzymology Molecular Sequence Data Molecular Weight Proinsulin/metabolism Proprotein Convertase 2 Proprotein Convertases Protein Processing, Post-Translational Rats Serine Endopeptidases/metabolism Substrate Specificity Subtilisins/metabolism
Chemicals
Proinsulin Proprotein Convertases Serine Endopeptidases Subtilisins Furin Proprotein Convertase 2
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Smeekens S P
Howard Hughes Medical Institute, Department of Biochemistry, University of Chicago, IL 60637.
Montag A G
Thomas G
Albiges-Rizo C
Carroll R
Benig M
Phillips L A
Martin S
Ohagi S
Gardner P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1992-09-15
Pages
8822-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50013
Subset
IM
Grants
NIDDK NIH HHS · DK 13914 · United States
NIDDK NIH HHS · DK 20595 · United States
NIDDK NIH HHS · DK 42086 · United States
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