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PMID: 1530878 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ornithine decarboxylase gene of Neurospora crassa: isolation, sequence, and polyamine-mediated regulation of its mRNA.

Molecular and cellular biology ·Vol. 12 ·No. 1 ·1992-01-00 ·Pages 347-59

Williams LJ, Barnett GR, Ristow JL, Pitkin J, Perriere M, Davis RH

Abstract

Ornithine decarboxylase (ODC), which initiates the biosynthesis of the polyamines putrescine, spermidine, and spermine, is encoded by the spe-1 gene of the fungus Neurospora crassa. This gene and its cDNA have been cloned and sequenced. The gene has a single 70-nucleotide intron in the coding sequence. The cDNA, comprising the entire coding region, recognizes a single 2.4-kb mRNA in Northern (RNA) blots. The mRNA transcript, defined by S1 mapping, has an extremely long, 535-base leader without strong secondary-structure features or an upstream reading frame. The translational start of the protein is ambiguous: a Met-Val-Met sequence precedes the Pro known to be the N terminus of the ODC polypeptide. The polypeptide encoded by the N. crassa spe-1 gene (484 amino acids) has 46% amino acid identity with that of Saccharomyces cerevisiae (466 amino acids) and 42% with that of mouse (461 amino acids). Alignment of the longer N. crassa sequence with S. cerevisiae and mouse sequences creates gaps in different sites in the S. cerevisiae and mouse sequences, suggesting that N. crassa ODC is closer to an ancestral form of the enzyme than that of either yeast or mouse ODC. N. crassa ODC, which turns over rapidly in vivo in the presence of polyamines, has two PEST sequences, found in most ODCs and other proteins with rapid turnover. In striking contrast to other eucaryotic organisms, the variation in the rate of ODC synthesis in response to polyamines in N. crassa is largely correlated with proportional changes in the abundance of ODC mRNA. Spermidine is the main effector of repression, while putrescine has a weaker effect. However, putrescine accumulation appears to increase the amount of active ODC that is made from a given amount of ODC mRNA, possibly by improving its translatability. Conversely, prolonged starvation for both putrescine and spermidine leads to the differentially impaired translation of ODC mRNA.

Related Genes
spe-1
MeSH Terms
Amino Acid Sequence Base Sequence DNA, Fungal/isolation & purification Gene Expression Regulation, Fungal Kinetics Molecular Sequence Data Neurospora crassa/enzymology,genetics Ornithine Decarboxylase/genetics,metabolism Polyamines/metabolism Putrescine/metabolism RNA Processing, Post-Transcriptional RNA, Fungal/isolation & purification,metabolism RNA, Messenger/isolation & purification,metabolism Restriction Mapping Spermidine/metabolism Transcription, Genetic
Chemicals
DNA, Fungal Polyamines RNA, Fungal RNA, Messenger Ornithine Decarboxylase Spermidine Putrescine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Williams L J
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Barnett G R
Ristow J L
Pitkin J
Perriere M
Davis R H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.1.347-359.1992
Published
1992-01-00
Pages
347-59
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364121
Subset
IM
Grants
NIGMS NIH HHS · GM-35120 · United States
NIGMS NIH HHS · GM07134 · United States
Databases
GENBANK
M68969, M68970
External Links
PubMed source DOI: 10.1128/mcb.12.1.347-359.1992 PMC Full Text View journal details
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