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PMID: 3456155 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Two ornithine decarboxylase mRNA species in mouse kidney arise from size heterogeneity at their 3' termini.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 3 ·1986-02-00 ·Pages 594-8

Hickok NJ, Seppänen PJ, Kontula KK, Jänne PA, Bardin CW, Jänne OA

Abstract

Ornithine decarboxylase (OrnDCase; L-ornithine carboxy-lyase, EC 4.1.1.17) mRNA present in mouse kidney comprises two species with molecular sizes of approximately 2.2 and approximately 2.7 kilobases (kb). cDNA clones prepared from murine kidney OrnDCase mRNA were used to determine the reason for the size heterogeneity of these mRNAs. Two of the cDNA clones (pODC16 and pODC74) that differed at the 3' termini were isolated and sequenced. DNA sequencing indicated that each cDNA had a poly(A) tail; however, pODC74 was 429 nucleotides longer than pODC16 at the 3' end and contained two AATAAA signals for poly(A) addition. That the longer cDNA corresponded to the larger mRNA was confirmed by hybridization of a unique Pst I/Pst I fragment from the 3' terminus of pODC74 only to the 2.7-kb OrnDCase mRNA. The two cDNAs did not represent full-length copies of OrnDCase mRNAs and were 1199 (pODC16) and 1204 base pairs (bp) (pODC74) long. There were five mismatches in their 759-bp-long overlapping nucleotide sequence, suggesting that the 2.2- and 2.7-kb OrnDCase mRNAs may be products of two separate, yet very similar, OrnDCase genes. Androgen regulation of the accumulation of these two OrnDCase mRNAs appeared to occur coordinately, as testosterone administration brought about comparable increases in their concentrations in mouse kidney.

MeSH Terms
Amino Acid Sequence Androgens/physiology Animals Base Sequence Cloning, Molecular DNA/genetics Female Kidney/enzymology Male Mice Ornithine Decarboxylase/biosynthesis,genetics RNA, Messenger/genetics
Chemicals
Androgens RNA, Messenger DNA Ornithine Decarboxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hickok N J
Seppänen P J
Kontula K K
Jänne P A
Bardin C W
Jänne O A
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.3.594
Published
1986-02-00
Pages
594-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322910
Subset
IM
Grants
NICHD NIH HHS · HD-13541 · United States
Databases
GENBANK
M12330, M12331
External Links
PubMed source DOI: 10.1073/pnas.83.3.594 PMC Full Text View journal details
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