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PMID: 7430252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Primary sequence of ovomucoid messenger RNA as determined from cloned complementary DNA.

The Journal of cell biology ·Vol. 87 ·No. 2 Pt 1 ·1980-11-00 ·Pages 480-7

Catterall JF, Stein JP, Kristo P, Means AR, O'Malley BW

Abstract

Ovomucoid messenger RNA (mRNAom) comprises approximately 8% of the total mRNA in the estrogen-stimulated oviduct. The recombinant plasmid pOM100 contained DNA complementary to the 3' end of mRNAom. DNA complementary to the 5' end of mRNAom was obtained from a partially purified preparation of mRNAom by polymerization by reverse transcriptase in the presence of a restriction fragment primer from pOM100. The complementary DNA mixture was amplified by molecular cloning using poly dG/dC tailing to form recombinant bacterial plasmids. Recombinant plasmids containing ovomucoid DNA sequences were selected by in situ hybridization to 32P-labeled pOM100 fragments. The longest plasmid containing ovomucoid DNA sequences was designated pOM502. The complete DNA sequence of both pOM100 and pOM502 was determined. The two plasmids appear to contain sequences complementary to the entire length of mRNAom. The nucleic acid sequence agrees with the known amino acid sequences for both ovomucoid and its N-terminal signal peptide. Highly homologous sequences occur in two regions that coincide with structural domains of the protein. Comparison of the sequence of mRNAom with that for other eucaryotic mRNAs allowed identification of possible functional regions in the mRNA molecule.

MeSH Terms
Animals Base Sequence Chickens Codon DNA, Recombinant Egg Proteins/genetics Nucleic Acid Conformation Ovomucin/genetics Plasmids Protein Biosynthesis Protein Conformation RNA, Messenger/genetics
Chemicals
Codon DNA, Recombinant Egg Proteins RNA, Messenger Ovomucin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Catterall J F
Stein J P
Kristo P
Means A R
O'Malley B W
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34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.87.2.480
Published
1980-11-00
Pages
480-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110750
Subset
IM
Grants
NICHD NIH HHS · HD07495 · United States
NICHD NIH HHS · HD08188 · United States
Databases
GENBANK
J00902
External Links
PubMed source DOI: 10.1083/jcb.87.2.480 PMC Full Text View journal details
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