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PMID: 15316114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of plant trichome development by a cotton fiber MYB gene.

The Plant cell ·Vol. 16 ·No. 9 ·2004-09-00 ·Pages 2323-34

Wang S, Wang JW, Yu N, Li CH, Luo B, Gou JY, Wang LJ, Chen XY

Abstract

Cotton (Gossypium spp) plants produce seed trichomes (cotton fibers) that are an important commodity worldwide; however, genes controlling cotton fiber development have not been characterized. In Arabidopsis thaliana the MYB gene GLABRA1 (GL1) is a central regulator of trichome development. Here, we show that promoter of a cotton fiber gene, RD22-like1 (RDL1), contains a homeodomain binding L1 box and a MYB binding motif that confer trichome-specific expression in Arabidopsis. A cotton MYB protein GaMYB2/Fiber Factor 1 transactivated the RDL1 promoter both in yeast and in planta. Real-time PCR and in situ analysis showed that GaMYB2 is predominantly expressed early in developing cotton fibers. After transferring into Arabidopsis, GL1::GaMYB2 rescued trichome formation of a gl1 mutant, and interestingly, 35S::GaMYB2 induced seed-trichome production. We further demonstrate that the first intron of both GL1 and GaMYB2 plays a role in patterning trichomes: it acts as an enhancer in trichome and a repressor in nontrichome cells, generating a trichome-specific pattern of MYB gene expression. Disruption of a MYB motif conserved in intron 1 of GL1, WEREWOLF, and GaMYB2 genes affected trichome production. These results suggest that cotton and Arabidopsis use similar transcription factors for regulating trichomes and that GaMYB2 may be a key regulator of cotton fiber development.

MeSH Terms
Amino Acid Motifs/genetics Enhancer Elements, Genetic/genetics Evolution, Molecular Gene Expression Regulation, Plant/genetics Gene Transfer Techniques Genes, myb/genetics,physiology Gossypium/genetics,growth & development,ultrastructure Introns/genetics Microscopy, Electron, Scanning Molecular Sequence Data Phylogeny Plants, Genetically Modified/genetics,growth & development,metabolism Promoter Regions, Genetic/genetics Protein Structure, Tertiary/genetics Repressor Proteins/genetics Seeds/genetics,growth & development,ultrastructure Transcription Factors/genetics,metabolism
Chemicals
Repressor Proteins Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wang Shui
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Wang Jia-Wei
Yu Nan
Li Chun-Hong
Luo Bin
Gou Jin-Ying
Wang Ling-Jian
Chen Xiao-Ya
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.024844
Published
2004-09-00
Epub
2004-00-17
Pages
2323-34
Language
English
Region
England
NLM ID
9208688
PMCID
PMC520936
Subset
IM
Databases
GENBANK
AY626159, AY626160, AY633558, AY633559, AY641990
External Links
PubMed source DOI: 10.1105/tpc.104.024844 PMC Full Text View journal details
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