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PMID: 15385622 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Oxidative stress activates FUS1 and RLM1 transcription in the yeast Saccharomyces cerevisiae in an oxidant-dependent Manner.

Molecular biology of the cell ·Vol. 15 ·No. 12 ·2004-12-00 ·Pages 5574-82

Staleva L, Hall A, Orlow SJ

Abstract

Mating in haploid Saccharomyces cerevisiae occurs after activation of the pheromone response pathway. Biochemical components of this pathway are involved in other yeast signal transduction networks. To understand more about the coordination between signaling pathways, we used a "chemical genetic" approach, searching for compounds that would activate the pheromone-responsive gene FUS1 and RLM1, a reporter for the cell integrity pathway. We found that catecholamines (l-3,4-hydroxyphenylalanine [l-dopa], dopamine, adrenaline, and noradrenaline) elevate FUS1 and RLM1 transcription. N-Acetyl-cysteine, a powerful antioxidant in yeast, completely reversed this effect, suggesting that FUS1 and RLM1 activation in response to catecholamines is a result of oxidative stress. The oxidant hydrogen peroxide also was found to activate transcription of an RLM1 reporter. Further genetic analysis combined with immunoblotting revealed that Kss1, one of the mating mitogen-activated protein kinases (MAPKs), and Mpk1, an MAPK of the cell integrity pathway, participated in l-dopa-induced stimulation of FUS1 and RLM1 transcription. We also report that Mpk1 and Hog1, the high osmolarity MAPK, were phosphorylated upon induction by hydrogen peroxide. Together, our results demonstrate that cells respond to oxidative stress via different signal transduction machinery dependent upon the nature of the oxidant.

MeSH Terms
Cell Membrane Permeability Gene Expression Regulation, Fungal/drug effects Hydrogen Peroxide/metabolism,pharmacology Levodopa/pharmacology MADS Domain Proteins MAP Kinase Signaling System/drug effects Membrane Proteins/genetics,metabolism Mitogen-Activated Protein Kinases/genetics,metabolism Mutation/genetics Oxidants/pharmacology Oxidative Stress/drug effects Pheromones/pharmacology Phosphorylation/drug effects Saccharomyces cerevisiae/drug effects,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic/drug effects
Chemicals
FUS1 protein, S cerevisiae MADS Domain Proteins Membrane Proteins Oxidants Pheromones RLM1 protein, S cerevisiae SLG1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Levodopa Hydrogen Peroxide FUS3 protein, S cerevisiae HOG1 protein, S cerevisiae KSS1 protein, S cerevisiae Mitogen-Activated Protein Kinases SLT2 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Staleva Liliana
Department of Dermatology, New York University School of Medicine, New York, NY 10016, USA.
Hall Andrea
Orlow Seth J
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-02-0142
Published
2004-12-00
Epub
2004-00-22
Pages
5574-82
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC532035
Subset
IM
Grants
NEI NIH HHS · R01 EY010223 · United States
NEI NIH HHS · EY10223 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-02-0142 PMC Full Text View journal details
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