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PMID: 15461800 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genome-wide mutagenesis of Zea mays L. using RescueMu transposons.

Genome biology ·Vol. 5 ·No. 10 ·2004-00-00 ·Pages R82

Fernandes J, Dong Q, Schneider B, Morrow DJ, Nan GL, Brendel V, Walbot V

Abstract

Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.

MeSH Terms
DNA Mutational Analysis DNA Transposable Elements/genetics DNA, Plant/genetics Gene Library Genes, Plant/genetics Genome, Plant Mutagenesis, Insertional/genetics,methods Phenotype Plasmids/genetics Zea mays/cytology,genetics
Chemicals
DNA Transposable Elements DNA, Plant
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fernandes John
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA. [email protected]
Dong Qunfeng
Schneider Bret
Morrow Darren J
Nan Guo-Ling
Brendel Volker
Walbot Virginia
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2004-5-10-r82
Email
[email protected]
Published
2004-00-00
Epub
2004-00-23
Pages
R82
Language
English
Region
England
NLM ID
100960660
PMCID
PMC545602
Subset
IM
External Links
PubMed source DOI: 10.1186/gb-2004-5-10-r82 PMC Full Text View journal details
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