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PMID: 9668130 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize.

The Plant cell ·Vol. 10 ·No. 7 ·1998-07-00 ·Pages 1095-1105

Hu G, Yalpani N, Briggs SP, Johal GS

Abstract

The maize lesion mimic gene Les22 is defined by dominant mutations and characterized by the production of minute necrotic spots on leaves in a developmentally specified and light-dependent manner. Phenotypically, Les22 lesions resemble those that are triggered during a hypersensitive disease resistance response of plants to pathogens. We have cloned Les22 by using a Mutator-tagging technique. It encodes uroporphyrinogen decarboxylase (UROD), a key enzyme in the biosynthetic pathway of chlorophyll and heme in plants. Urod mutations in humans are also dominant and cause the metabolic disorder porphyria, which manifests itself as light-induced skin morbidity resulting from an excessive accumulation of photoexcitable uroporphyrin. The phenotypic and genetic similarities between porphyria and Les22 along with our observation that Les22 is also associated with an accumulation of uroporphyrin revealed what appears to be a case of natural porphyria in plants.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Plant/chemistry,genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genes, Plant Homozygote Hordeum/enzymology Light Molecular Sequence Data Mutation Phenotype Plant Diseases/genetics Plants, Toxic Porphyrins/metabolism Sequence Alignment Sequence Homology, Amino Acid Tobacco/enzymology Uroporphyrinogen Decarboxylase/biosynthesis,chemistry,genetics Zea mays/enzymology,genetics,growth & development
Chemicals
DNA, Plant Porphyrins Uroporphyrinogen Decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hu G
Department of Agronomy, University of Missouri, Columbia, Missouri 65211, USA.
Yalpani N
Briggs S P
Johal G S
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.7.1095
Published
1998-07-00
Pages
1095-1105
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144048
Subset
IM
Corrections
CommentIn
PMID: 9668127
External Links
PubMed source DOI: 10.1105/tpc.10.7.1095 PMC Full Text View journal details
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