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PMID: 15545640 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Bonus, a Drosophila TIF1 homolog, is a chromatin-associated protein that acts as a modifier of position-effect variegation.

Genetics ·Vol. 169 ·No. 2 ·2005-02-00 ·Pages 783-94

Beckstead RB, Ner SS, Hales KG, Grigliatti TA, Baker BS, Bellen HJ

Abstract

Bonus, a Drosophila TIF1 homolog, is a nuclear receptor cofactor required for viability, molting, and numerous morphological events. Here we establish a role for Bonus in the modulation of chromatin structure. We show that weak loss-of-function alleles of bonus have a more deleterious effect on males than on females. This male-enhanced lethality is not due to a defect in dosage compensation or somatic sex differentiation, but to the presence of the Y chromosome. Additionally, we show that bonus acts as both an enhancer and a suppressor of position-effect variegation. By immunostaining, we demonstrate that Bonus is associated with both interphase and prophase chromosomes and through chromatin immunoprecipitation show that two of these sites correspond to the histone gene cluster and the Stellate locus.

Keywords
NASA Discipline Developmental Biology Non-NASA Center
MeSH Terms
Animals Chromatin/genetics,metabolism Chromatin Immunoprecipitation Drosophila/genetics,metabolism Drosophila Proteins/genetics,metabolism Enhancer Elements, Genetic Female Gene Expression Regulation Genes, Insect Immunohistochemistry Male Microscopy, Confocal Mutation Nuclear Proteins/genetics,metabolism Receptors, Cytoplasmic and Nuclear/genetics,metabolism Repressor Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Y Chromosome
Chemicals
Bon protein, Drosophila Chromatin Drosophila Proteins Nuclear Proteins Receptors, Cytoplasmic and Nuclear Repressor Proteins Transcription Factors transcriptional intermediary factor 1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Beckstead R B
Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA.
Ner S S
Hales K G
Grigliatti T A
Baker B S
Bellen H J
Investigators
1 investigators, click to expand
Bellen H J
Baylor Coll Med, Houston, TX
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.037085
Published
2005-02-00
Epub
2004-00-15
Pages
783-94
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1449102
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.104.037085 PMC Full Text View journal details
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