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PMID: 15604691 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis whole-transcriptome profiling defines the features of coordinated regulations that occur during secondary growth.

Plant molecular biology ·Vol. 55 ·No. 3 ·2004-05-00 ·Pages 433-53

Ko JH, Han KH

Abstract

Secondary growth in the inflorescence stems of Arabidopsis plants was induced by a combination of short-day and long-day treatments. The induced stems were divided into three different stem developmental stages (i.e., immature, intermediate, and mature) with regard to secondary growth. Whole transcriptome microarrays were used to examine the changes in global gene expression occurring at the different stem developmental stages. Over 70% of the Arabidopsis transcriptome was expressed in the stem tissues. In the mature stems with secondary growth, 567 genes were upregulated 5-fold or higher and 530 were downregulated, when compared to immature stems (with no secondary growth) and 10-day old seedlings (with no inflorescence stem). The transcription phenotypes obtained from the stems at different developmental stages largely confirm the existing insights into the biochemical processes involved in the sequential events that lead to wood formation. The major difference found between the stems undergoing secondary growth and only primary growth was in the expression profiles of transcriptional regulation-and signal transduction-related genes. An analysis of several shoot apical meristem (SAM) activity-related gene expression patterns in the stems indicated that the genetic control of secondary meristem activity might be governed by a different mechanism from that of SAM. The current study established the expression patterns of many unknown genes and identified candidate genes that are involved in the genetic regulation of secondary growth. The findings described in this report should improve our understanding of the molecular mechanisms that regulate the growth and development of the stem.

MeSH Terms
Apoptosis/genetics Arabidopsis/genetics,growth & development,radiation effects Cell Division/genetics Cluster Analysis Flowers/genetics,growth & development,radiation effects Gene Expression Profiling Gene Expression Regulation, Developmental/radiation effects Gene Expression Regulation, Plant/radiation effects Meristem/genetics,growth & development,radiation effects Oligonucleotide Array Sequence Analysis/methods Photoperiod Plant Stems/genetics,growth & development,radiation effects Transcription, Genetic/genetics,radiation effects
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ko Jae-Heung
Department of Forestry, Michigan State University, 126 Natural Resources, MI 48824-1222, USA.
Han Kyung-Hwan
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-004-1051-z
Published
2004-05-00
Pages
433-53
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-004-1051-z View journal details
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