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PMID: 15608335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication.

The Plant cell ·Vol. 17 ·No. 1 ·2005-01-00 ·Pages 311-25

Selth LA, Dogra SC, Rasheed MS, Healy H, Randles JW, Rezaian MA

Abstract

Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Nucleus/genetics,metabolism DNA, Viral/genetics Evolution, Molecular Gene Expression Regulation, Plant/genetics Gene Expression Regulation, Viral/genetics Lycopersicon esculentum/genetics,metabolism Molecular Sequence Data Phylogeny Plant Diseases/virology Plant Proteins/genetics,isolation & purification,metabolism Plant Viruses/genetics,metabolism Protein Structure, Tertiary/physiology Transcription Factors/genetics,isolation & purification,metabolism Transcriptional Activation/genetics Up-Regulation/genetics Viral Proteins/genetics,metabolism Virus Replication/genetics
Chemicals
DNA, Viral Plant Proteins Transcription Factors Viral Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Selth Luke A
Horticulture Unit, Commonwealth Scientific and Industrial Research Organization, Plant Industry, Glen Osmond, South Australia, 5064, Australia.
Dogra Satish C
Rasheed M Saif
Healy Helen
Randles John W
Rezaian M Ali
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.027235
Published
2005-01-00
Epub
2004-00-17
Pages
311-25
Language
English
Region
England
NLM ID
9208688
PMCID
PMC544507
Subset
IM
Databases
GENBANK
AY498713, AY622990
External Links
PubMed source DOI: 10.1105/tpc.104.027235 PMC Full Text View journal details
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