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PMID: 10880461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants.

The EMBO journal ·Vol. 19 ·No. 13 ·2000-07-03 ·Pages 3485-95

Kong LJ, Orozco BM, Roe JL, Nagar S, Ou S, Feiler HS, Durfee T, Miller AB, Gruissem W, Robertson D, Hanley-Bowdoin L

Abstract

Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homo logues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1-pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1-pRBR interactions during geminivirus infection of plants.

Keywords
Non-programmatic
MeSH Terms
Antigens, Polyomavirus Transforming/metabolism Base Sequence DNA Primers Geminiviridae/isolation & purification,metabolism,physiology Plants/virology Protein Binding Retinoblastoma Protein/metabolism Viral Proteins/metabolism Virus Replication
Chemicals
Antigens, Polyomavirus Transforming DNA Primers Retinoblastoma Protein Viral Proteins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Kong L J
Departments of Biochemistry and Botany, North Carolina State University, Raleigh, NC 27695, USA.
Orozco B M
Roe J L
Nagar S
Ou S
Feiler H S
Durfee T
Miller A B
Gruissem W
Robertson D
Hanley-Bowdoin L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.13.3485
Published
2000-07-03
Pages
3485-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC313951
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.13.3485 PMC Full Text View journal details
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