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PMID: 9862488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis.

Plant molecular biology ·Vol. 38 ·No. 5 ·1998-11-00 ·Pages 699-712

Horváth GV, Pettkó-Szandtner A, Nikovics K, Bilgin M, Boulton M, Davies JW, Gutiérrez C, Dudits D

Abstract

The replication of the geminiviruses depends on the viral encoded early (complementary-sense) proteins and on host genome encoded factors. Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.

MeSH Terms
Amino Acid Sequence Binding Sites Cell Cycle Proteins Consensus Sequence DNA Helicases/genetics,metabolism DNA-Binding Proteins Dimerization Geminiviridae/genetics,physiology Plant Proteins/genetics,metabolism Protein Binding Proteins/genetics,metabolism Retinoblastoma Protein/genetics Trans-Activators/genetics,metabolism Viral Proteins/chemistry,genetics,metabolism Virus Replication Zea mays/genetics,metabolism
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Plant Proteins Proteins RRB1 protein, Zea mays Retinoblastoma Protein Trans-Activators Viral Proteins replication initiator protein DNA Helicases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Horváth G V
Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged.
Pettkó-Szandtner A
Nikovics K
Bilgin M
Boulton M
Davies J W
Gutiérrez C
Dudits D
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1006076316887
Published
1998-11-00
Pages
699-712
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1006076316887 View journal details
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