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PMID: 15618420 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts.

Plant physiology ·Vol. 137 ·No. 1 ·2005-01-00 ·Pages 104-16

Borner GH, Sherrier DJ, Weimar T, Michaelson LV, Hawkins ND, Macaskill A, Napier JA, Beale MH, Lilley KS, Dupree P

Abstract

The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- and sterol-rich membrane domains. The aim of this work was to obtain evidence for lipid domain phenomena in plant membranes. A protocol to prepare Triton X-100 detergent-resistant membranes (DRMs) was developed using Arabidopsis (Arabidopsis thaliana) callus membranes. A comparative proteomics approach using two-dimensional difference gel electrophoresis and liquid chromatography-tandem mass spectrometry revealed that the DRMs were highly enriched in specific proteins. They included eight glycosylphosphatidylinositol-anchored proteins, several plasma membrane (PM) ATPases, multidrug resistance proteins, and proteins of the stomatin/prohibitin/hypersensitive response family, suggesting that the DRMs originated from PM domains. We also identified a plant homolog of flotillin, a major mammalian DRM protein, suggesting a conserved role for this protein in lipid domain phenomena in eukaryotic cells. Lipid analysis by gas chromatography-mass spectrometry showed that the DRMs had a 4-fold higher sterol-to-protein content than the average for Arabidopsis membranes. The DRMs were also 5-fold increased in sphingolipid-to-protein ratio. Our results indicate that the preparation of DRMs can yield a very specific set of membrane proteins and suggest that the PM contains phytosterol and sphingolipid-rich lipid domains with a specialized protein composition. Our results also suggest a conserved role of lipid modification in targeting proteins to both the intracellular and extracellular leaflet of these domains. The proteins associated with these domains provide important new experimental avenues into understanding plant cell polarity and cell surface processes.

MeSH Terms
Arabidopsis/chemistry,ultrastructure Detergents Gene Expression Membrane Microdomains/chemistry Proteomics
Chemicals
Detergents
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Borner Georg H H
Department of Biochemistry , University of Cambridge, Cambridge CB2 1QW, UK.
Sherrier D Janine
Weimar Thilo
Michaelson Louise V
Hawkins Nathan D
Macaskill Andrew
Napier Johnathan A
Beale Michael H
Lilley Kathryn S
Dupree Paul
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.104.053041
Published
2005-01-00
Epub
2004-00-23
Pages
104-16
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC548842
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/C507561/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · G18889 · United Kingdom
Corrections
CommentIn
PMID: 17344433
External Links
PubMed source DOI: 10.1104/pp.104.053041 PMC Full Text View journal details
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